@article{f5cd71b5a0784a299cd48be7f243d284,
title = "Multimodal Analysis of Cell Types in a Hypothalamic Node Controlling Social Behavior",
abstract = "Single-cell RNA sequencing of the mouse ventrolateral subdivision of the ventromedial hypothalamus, a brain region that contains ∼4,000 neurons and controls innate social behaviors including aggression and mounting, reveals several transcriptomic cell types that differ between males and females.",
keywords = "VMH, aggression, cell types, estrogen receptor, hypothalamus, mating, metabolism, sexual dimorphism, single-cell RNA sequencing, social behavior",
author = "Kim, {Dong Wook} and Zizhen Yao and Graybuck, {Lucas T.} and Kim, {Tae Kyung} and Nguyen, {Thuc Nghi} and Smith, {Kimberly A.} and Olivia Fong and Lynn Yi and Noushin Koulena and Nico Pierson and Sheel Shah and Liching Lo and Pool, {Allan Hermann} and Yuki Oka and Lior Pachter and Long Cai and Bosiljka Tasic and Hongkui Zeng and Anderson, {David J.}",
note = "Funding Information: We thank A. Jones and C. Koch for support at the Allen Institute for Brain Sciences during the writing of this manuscript, J.-S. Chang for cell counting, Y. Huang for genotyping, G. Mancuso for administrative assistance, C. Chiu for lab management, S. Diamond for assistance with FACS, the Single Cell Profiling and Engineering Center (SPEC) in the Beckman Institute at Caltech for initial help for 10x scRNA-seq experiments, S. Pease for assistance with transgenic mouse strains, J. Costanza for mouse colony management, members of the Anderson laboratory for helpful comments on this project, and an anonymous reviewer for suggesting social fear testing in group-housed mice. This work was supported by US National Institutes of Health (NIH) BRAIN Initiative grants U01MH105982 and U19MH114830 to H.Z. and D.J.A. and NIH grants MH070053 and TR01 OD024686 to D.J.A. and L.C. respectively. D.-W.K. was supported by a Howard Hughes Medical Institute International Student Research Fellowship. D.J.A. is an investigator of the Howard Hughes Medical Institute. D.-W.K. B.T. H.Z. and D.J.A. contributed to the study design. D.-W.K. performed most of the experiments. T.K.K. and K.A.S. prepared sequencing libraries for SMART-seq scRNA-seq. L.T.G and O.F. contributed data visualization. D.-W.K. and T.N.N performed Retro-seq experiments. D.-W.K. Z.Y, L.T.G, L.Y. and L.P. analyzed the scRNA-seq data. D.-W.K. and N.K. performed seqFISH experiments. D.-W.K. N.P. S.S. and L.C. analyzed the seqFISH data. D.-W.K. and L.L. performed retrograde labeling with c-fos immunohistochemistry experiments. A.-H.P. and Y.O. developed the tissue preparation protocols for 10x Act-seq. D.J.A. supervised the project. D.-W.K. and D.J.A. wrote the manuscript with contributions from B.T. and H.Z. All authors discussed and commented on the manuscript. The authors declare no competing interests. Funding Information: We thank A. Jones and C. Koch for support at the Allen Institute for Brain Sciences during the writing of this manuscript, J.-S. Chang for cell counting, Y. Huang for genotyping, G. Mancuso for administrative assistance, C. Chiu for lab management, S. Diamond for assistance with FACS, the Single Cell Profiling and Engineering Center (SPEC) in the Beckman Institute at Caltech for initial help for 10x scRNA-seq experiments, S. Pease for assistance with transgenic mouse strains, J. Costanza for mouse colony management, members of the Anderson laboratory for helpful comments on this project, and an anonymous reviewer for suggesting social fear testing in group-housed mice. This work was supported by US National Institutes of Health (NIH) BRAIN Initiative grants U01MH105982 and U19MH114830 to H.Z. and D.J.A. and NIH grants MH070053 and TR01 OD024686 to D.J.A. and L.C., respectively. D.-W.K. was supported by a Howard Hughes Medical Institute International Student Research Fellowship. D.J.A. is an investigator of the Howard Hughes Medical Institute. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
month = oct,
day = "17",
doi = "10.1016/j.cell.2019.09.020",
language = "English (US)",
volume = "179",
pages = "713--728.e17",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3",
}