Abstract
While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.
Original language | English (US) |
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Article number | 030505 |
Journal | Journal of biomedical optics |
Volume | 15 |
Issue number | 3 |
DOIs | |
State | Published - May 2010 |
Externally published | Yes |
Keywords
- Near-infrared imaging
- Two-photon microscopy
- Ultrafast fiber lasers
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Biomaterials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering