Multiphoton microscopy with near infrared contrast agents

Siavash Yazdanfar, Chulmin Joo, Chun Zhan, Mikhail Y. Berezin, Walter J. Akers, Samuel Achilefu

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

While multiphoton microscopy (MPM) has been performed with a wide range of excitation wavelengths, fluorescence emission has been limited to the visible spectrum. We introduce a paradigm for MPM of near-infrared (NIR) fluorescent molecular probes via nonlinear excitation at 1550 nm. This all-NIR system expands the range of available MPM fluorophores, virtually eliminates background autofluorescence, and allows for use of fiber-based, turnkey ultrafast lasers developed for telecommunications.

Original languageEnglish (US)
Article number030505
JournalJournal of biomedical optics
Volume15
Issue number3
DOIs
StatePublished - May 2010
Externally publishedYes

Keywords

  • Near-infrared imaging
  • Two-photon microscopy
  • Ultrafast fiber lasers

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

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