Abstract
Most 13C nuclear magnetic resonance (NMR) isotopomer analyses relate a metabolic index of interest to populations of 13C isotopomers as reported by one-bond 13C-13C spin-spin couplings. Metabolic conditions that produce highly enriched citric acid cycle intermediates often lead to 13C NMR spectra of metabolites such as glutamate that show extra multiplets due to long-range couplings. It can be demonstrated from 13C NMR spectra of hearts perfused with mixtures of acetate plus propionate that multiplets in glutamate C2 arising from 3J25 coupling provide a direct readout of acetyl- CoA fractional enrichment (F(c1) and F(c3)), while multiplets in glutamate C5 arising from 2J35 and 3J25 couplings quantitatively reflect enrichment of the anaplerotic substrate.
Original language | English (US) |
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Pages (from-to) | 197-200 |
Number of pages | 4 |
Journal | Magnetic resonance in medicine |
Volume | 42 |
Issue number | 1 |
DOIs | |
State | Published - 1999 |
Keywords
- C NMR
- Isotopomer analysis
- Long-range coupling
ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging