Multiple bond 13C-13C spin-spin coupling provides complementary information in a 13C NMR isotopomer analysis of glutamate

Rui A. Carvalho, Evelyn E. Babcock, F. Mark H Jeffrey, A. Dean Sherry, Craig R. Malloy

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Most 13C nuclear magnetic resonance (NMR) isotopomer analyses relate a metabolic index of interest to populations of 13C isotopomers as reported by one-bond 13C-13C spin-spin couplings. Metabolic conditions that produce highly enriched citric acid cycle intermediates often lead to 13C NMR spectra of metabolites such as glutamate that show extra multiplets due to long-range couplings. It can be demonstrated from 13C NMR spectra of hearts perfused with mixtures of acetate plus propionate that multiplets in glutamate C2 arising from 3J25 coupling provide a direct readout of acetyl- CoA fractional enrichment (F(c1) and F(c3)), while multiplets in glutamate C5 arising from 2J35 and 3J25 couplings quantitatively reflect enrichment of the anaplerotic substrate.

Original languageEnglish (US)
Pages (from-to)197-200
Number of pages4
JournalMagnetic resonance in medicine
Issue number1
StatePublished - 1999


  • C NMR
  • Isotopomer analysis
  • Long-range coupling

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging


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