Multiple cancer testis antigens function to support tumor cell mitotic fidelity

Kathryn M. Cappell, Rebecca Sinnott, Patrick Taus, Kimberly Maxfield, Moriah Scarbrough, Angelique W. Whitehurst

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

While the expression of genes that are normally involved in spermatogenesis is frequently detected in tumors, the extent to which these gene products are required for neoplastic behaviors is unclear. To begin to address their functional relevance to tumorigenesis, we identified a cohort of proteins which display synthetic lethality with paclitaxel in non-small-cell lung cancer and whose expression is biased toward testes and tumors. Remarkably, these testis proteins, FMR1NB, NXF2, MAGEA5, FSIP1, and STARD6, are required for accurate chromosome segregation in tumor cells. Their individual depletion enhances the generation of multipolar spindles, increases mitotic transit time, and induces micronucleation in response to an otherwise innocuous dose of paclitaxel. The underlying basis for abnormal mitosis is an alteration in microtubule function, as their depletion increases microtubule cytaster formation and disrupts microtubule stability. Given these observations, we hypothesize that reactivated testis proteins may represent unique tumor cell vulnerabilities which, if targeted, could enhance responsiveness to antimitotic therapy. Indeed, we demonstrate that combining paclitaxel with a small-molecule inhibitor of the gametogenic and tumor cell mitotic protein TACC3 leads to enhanced centrosomal abnormalities, activation of death programs, and loss of anchorage-independent growth.

Original languageEnglish (US)
Pages (from-to)4131-4140
Number of pages10
JournalMolecular and Cellular Biology
Volume32
Issue number20
DOIs
StatePublished - Oct 2012

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Testicular Neoplasms
Antigens
Paclitaxel
Microtubules
Testis
Neoplasms
Proteins
Antimitotic Agents
Chromosome Segregation
Spindle Apparatus
Spermatogenesis
Mitosis
Non-Small Cell Lung Carcinoma
Carcinogenesis
Gene Expression
Growth
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Multiple cancer testis antigens function to support tumor cell mitotic fidelity. / Cappell, Kathryn M.; Sinnott, Rebecca; Taus, Patrick; Maxfield, Kimberly; Scarbrough, Moriah; Whitehurst, Angelique W.

In: Molecular and Cellular Biology, Vol. 32, No. 20, 10.2012, p. 4131-4140.

Research output: Contribution to journalArticle

Cappell, Kathryn M. ; Sinnott, Rebecca ; Taus, Patrick ; Maxfield, Kimberly ; Scarbrough, Moriah ; Whitehurst, Angelique W. / Multiple cancer testis antigens function to support tumor cell mitotic fidelity. In: Molecular and Cellular Biology. 2012 ; Vol. 32, No. 20. pp. 4131-4140.
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