TY - JOUR
T1 - Multiple model approach--dealing with alignment ambiguities in protein modeling.
AU - Pawłowski, K.
AU - Jaroszewski, L.
AU - Bierzyñski, A.
AU - Godzik, A.
N1 - Copyright:
This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
PY - 1997
Y1 - 1997
N2 - Sequence alignments for distantly homologous proteins are often ambiguous, which creates a weak link in structure prediction by homology. We address this problem by using several plausible alignments in a modeling procedure, obtaining many models of the target. All are subsequently evaluated by a threading algorithm. It is shown that this approach can identify best alignments and produce reasonable models, whose quality is now limited only by the extent of the structural similarity between the known and predicted protein. Using a similar approach structure prediction for the oxidized dimer of S100A1 protein, for which the structure is not known, is presented.
AB - Sequence alignments for distantly homologous proteins are often ambiguous, which creates a weak link in structure prediction by homology. We address this problem by using several plausible alignments in a modeling procedure, obtaining many models of the target. All are subsequently evaluated by a threading algorithm. It is shown that this approach can identify best alignments and produce reasonable models, whose quality is now limited only by the extent of the structural similarity between the known and predicted protein. Using a similar approach structure prediction for the oxidized dimer of S100A1 protein, for which the structure is not known, is presented.
UR - http://www.scopus.com/inward/record.url?scp=0031300844&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031300844&partnerID=8YFLogxK
M3 - Article
C2 - 9390303
AN - SCOPUS:0031300844
SP - 328
EP - 339
JO - Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing
JF - Pacific Symposium on Biocomputing. Pacific Symposium on Biocomputing
SN - 2335-6936
ER -