Multispectral and photoplethysmography optical imaging techniques identify important tissue characteristics in an animal model of tangential burn excision

Jeffrey E. Thatcher, Weizhi Li, Yolanda Rodriguez-Vaqueiro, John J. Squiers, Weirong Mo, Yang Lu, Kevin D. Plant, Eric Sellke, Darlene R. King, Wensheng Fan, Jose A. Martinez-Lorenzo, J. Michael DiMaio

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Burn excision, a difficult technique owing to the training required to identify the extent and depth of injury, will benefit from a tool that can cue the surgeon as to where and how much to resect. We explored two rapid and noninvasive optical imaging techniques in their ability to identify burn tissue from the viable wound bed using an animal model of tangential burn excision. Photoplethysmography (PPG) imaging and multispectral imaging (MSI) were used to image the initial, intermediate, and final stages of burn excision of a deep partial-thickness burn. PPG imaging maps blood flow in the skin's microcirculation, and MSI collects the tissue reflectance spectrum in visible and infrared wavelengths of light to classify tissue based on a reference library. A porcine deep partial-thickness burn model was generated and serial tangential excision accomplished with an electric dermatome set to 1.0 mm depth. Excised eschar was stained with hematoxylin and eosin to determine the extent of burn remaining at each excision depth. We confirmed that the PPG imaging device showed significantly less blood flow where burn tissue was present, and the MSI method could delineate burn tissue in the wound bed from the viable wound bed. These results were confirmed independently by a histological analysis. We found these devices can identify the proper depth of excision, and their images could cue a surgeon as to the preparedness of the wound bed for grafting. These image outputs are expected to facilitate clinical judgment in the operating room.

Original languageEnglish (US)
Pages (from-to)38-52
Number of pages15
JournalJournal of Burn Care and Research
Volume37
Issue number1
DOIs
StatePublished - 2016

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Photoplethysmography
Optical Imaging
Animal Models
Wounds and Injuries
Cues
Equipment and Supplies
Aptitude
Operating Rooms
Hematoxylin
Eosine Yellowish-(YS)
Microcirculation
Libraries
Swine
Light
Skin

ASJC Scopus subject areas

  • Emergency Medicine
  • Rehabilitation
  • Surgery

Cite this

Multispectral and photoplethysmography optical imaging techniques identify important tissue characteristics in an animal model of tangential burn excision. / Thatcher, Jeffrey E.; Li, Weizhi; Rodriguez-Vaqueiro, Yolanda; Squiers, John J.; Mo, Weirong; Lu, Yang; Plant, Kevin D.; Sellke, Eric; King, Darlene R.; Fan, Wensheng; Martinez-Lorenzo, Jose A.; Michael DiMaio, J.

In: Journal of Burn Care and Research, Vol. 37, No. 1, 2016, p. 38-52.

Research output: Contribution to journalArticle

Thatcher, JE, Li, W, Rodriguez-Vaqueiro, Y, Squiers, JJ, Mo, W, Lu, Y, Plant, KD, Sellke, E, King, DR, Fan, W, Martinez-Lorenzo, JA & Michael DiMaio, J 2016, 'Multispectral and photoplethysmography optical imaging techniques identify important tissue characteristics in an animal model of tangential burn excision', Journal of Burn Care and Research, vol. 37, no. 1, pp. 38-52. https://doi.org/10.1097/BCR.0000000000000317
Thatcher, Jeffrey E. ; Li, Weizhi ; Rodriguez-Vaqueiro, Yolanda ; Squiers, John J. ; Mo, Weirong ; Lu, Yang ; Plant, Kevin D. ; Sellke, Eric ; King, Darlene R. ; Fan, Wensheng ; Martinez-Lorenzo, Jose A. ; Michael DiMaio, J. / Multispectral and photoplethysmography optical imaging techniques identify important tissue characteristics in an animal model of tangential burn excision. In: Journal of Burn Care and Research. 2016 ; Vol. 37, No. 1. pp. 38-52.
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