TY - JOUR
T1 - Multispectral imaging in the extended near-infrared window based on endogenous chromophores
AU - Cao, Qian
AU - Zhegalova, Natalia G.
AU - Wang, Steven T.
AU - Akers, Walter J.
AU - Berezin, Mikhail Y.
N1 - Funding Information:
We gratefully acknowledge financial support from the National Cancer Institute of the National Institutes of Health (R21CA149814), the National Heart Lung and Blood Institute as a Program of Excellence in Nanotechnology (HHSN268201000046C) and funds provided by the Washington University Molecular Imaging Center. We also thank funds provided by the Mallinckrodt Institute of Radiology Summer Research Program (Q.C.).
PY - 2013
Y1 - 2013
N2 - To minimize the problem with scattering in deep tissues while increasing the penetration depth, we explored the feasibility of imaging in the relatively unexplored extended near infrared (exNIR) spectral region at 900 to 1400nm with endogenous chromophores. This region, also known as the second NIR window, is weakly dominated by absorption from water and lipids and is free from other endogenous chromophores with virtually no autofluorescence. To demonstrate the applicability of the exNIR for bioimaging, we analyzed the optical properties of individual components and biological tissues using an InGaAs spectrophotometer and a multispectral InGaAs scanning imager featuring transmission geometry. Based on the differences in spectral properties of tissues, we utilized ratiometric approaches to extract spectral characteristics from the acquired three-dimensional "datacube". The obtained images of an exNIR transmission through a mouse head revealed sufficient details consistent with anatomical structures.
AB - To minimize the problem with scattering in deep tissues while increasing the penetration depth, we explored the feasibility of imaging in the relatively unexplored extended near infrared (exNIR) spectral region at 900 to 1400nm with endogenous chromophores. This region, also known as the second NIR window, is weakly dominated by absorption from water and lipids and is free from other endogenous chromophores with virtually no autofluorescence. To demonstrate the applicability of the exNIR for bioimaging, we analyzed the optical properties of individual components and biological tissues using an InGaAs spectrophotometer and a multispectral InGaAs scanning imager featuring transmission geometry. Based on the differences in spectral properties of tissues, we utilized ratiometric approaches to extract spectral characteristics from the acquired three-dimensional "datacube". The obtained images of an exNIR transmission through a mouse head revealed sufficient details consistent with anatomical structures.
KW - extended near infrared multispectral imaging endogenous chromophores water lipids
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U2 - 10.1117/1.JBO.18.10.101318
DO - 10.1117/1.JBO.18.10.101318
M3 - Article
C2 - 23933967
AN - SCOPUS:84886792063
SN - 1083-3668
VL - 18
JO - Journal of biomedical optics
JF - Journal of biomedical optics
IS - 10
M1 - 101318
ER -