Multistage Progression of Abelson Virus-infected Murine Pre-B-Cells to the Tumorigenic State

Gregory J. Palumbo, Brad W. Ozanne, John R. Kettman

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The tumorigenic potential of pre-B-cells at different stages of Abelson murine leukemia virus-induced transformation was determined. Cell lines with low growth potential in liquid culture were found (a) to have a dose-dependent growth requirement for conditioned medium obtained from bone marrow cultures, (b) to have low colony-forming ability in semisolid medium in the absence of conditioned medium, and (c) to be nontumorigenic when inoculated into syngeneic mice. Culture of the factor-dependent cells in vitro leads to the emergence of factor-independent variants, which eventually dominate the population by overgrowth. Cell lines that acquired a factor-independent phenotype were able to form colonies in semisolid medium and form tumors when inoculated into syngeneic mice. These results suggest that Abelson murine leukemia virus is sufficient to initiate transformation in the infected cell but that an additional genetic alteration is needed to confer tumorigenicity.

Original languageEnglish (US)
Pages (from-to)1917-1923
Number of pages7
JournalCancer research
Volume50
Issue number6
StatePublished - Mar 15 1990

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Multistage Progression of Abelson Virus-infected Murine Pre-B-Cells to the Tumorigenic State'. Together they form a unique fingerprint.

Cite this