MUNC, a long noncoding RNA that facilitates the function of MyoD in skeletal myogenesis

Adam C. Mueller, Magdalena A. Cichewicz, Bijan K. Dey, Ryan Layer, Brian J. Reon, Jeffrey R. Gagan, Anindya Dutta

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

An in silico screen for myogenic long noncoding RNAs (lncRNAs) revealed nine lncRNAs that are upregulated more than 10-fold in myotubes versus levels in myoblasts. One of these lncRNAs, MyoD upstream noncoding (MUNC, also known as DRReRNA), is encoded 5 kb upstream of the transcription start site of MyoD, a myogenic transcription factor gene. MUNC is specifically expressed in skeletal muscle and exists as in unspliced and spliced isoforms, and its 5' end overlaps with the cis-acting distal regulatory region (DRR) of MyoD. Small interfering RNA (siRNA) of MUNC reduced myoblast differentiation and specifically reduced the association of MyoD to the DRR enhancer and myogenin promoter but not to another MyoD-dependent enhancer. Stable overexpression of MUNC from a heterologous promoter increased endogenous MyoD, Myogenin, and Myh3 (myosin heavy chain, [MHC] gene) mRNAs but not the cognate proteins, suggesting that MUNC can act in trans to promote gene expression but that this activity does not require an induction of MyoD protein. MUNC also stimulates the transcription of other genes that are not recognized as MyoD-inducible genes. Knockdown of MUNC in vivo impaired murine muscle regeneration, implicating MUNC in primary satellite cell differentiation in the animal. We also discovered a human MUNC that is induced during differentiation of myoblasts and whose knockdown decreases differentiation, suggesting an evolutionarily conserved role of MUNC lncRNA in myogenesis. Although MUNC overlaps with the DRR enhancer, our results suggest that MUNC is not a classic cisacting enhancer RNA (e-RNA) acting exclusively by stimulating the neighboring MyoD gene but more like a promyogenic lncRNA that acts directly or indirectly on multiple promoters to increase myogenic gene expression.

Original languageEnglish (US)
Pages (from-to)498-513
Number of pages16
JournalMolecular and cellular biology
Volume35
Issue number3
DOIs
StatePublished - Jan 1 2015
Externally publishedYes

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Long Noncoding RNA
Muscle Development
Myoblasts
Nucleic Acid Regulatory Sequences
Myogenin
Genes
MyoD Protein
Gene Expression
Myosin Heavy Chains
Transcription Initiation Site
Skeletal Muscle Fibers
Computer Simulation
Small Interfering RNA
Regeneration
Cell Differentiation
Protein Isoforms
Skeletal Muscle
Transcription Factors
RNA
Muscles

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

MUNC, a long noncoding RNA that facilitates the function of MyoD in skeletal myogenesis. / Mueller, Adam C.; Cichewicz, Magdalena A.; Dey, Bijan K.; Layer, Ryan; Reon, Brian J.; Gagan, Jeffrey R.; Dutta, Anindya.

In: Molecular and cellular biology, Vol. 35, No. 3, 01.01.2015, p. 498-513.

Research output: Contribution to journalArticle

Mueller, Adam C. ; Cichewicz, Magdalena A. ; Dey, Bijan K. ; Layer, Ryan ; Reon, Brian J. ; Gagan, Jeffrey R. ; Dutta, Anindya. / MUNC, a long noncoding RNA that facilitates the function of MyoD in skeletal myogenesis. In: Molecular and cellular biology. 2015 ; Vol. 35, No. 3. pp. 498-513.
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