Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols)

S. M. Robertson, J. R. Kettman, J. N. Miller, M. V. Norgard

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Murine anti-Treponema pallidum (Nichols) lymphocyte hybridoma cell lines secreting monoclonal antibodies against a variety of treponemal antigens have been generated. Hybridomas isolated were of three major types: those that were directed specifically against T. pallidum antigens, those that were directed against treponemal group antigens (as evidenced by their cross-reactivity with T. phagedenis biotype Reiter antigens), and those that cross-reacted with both treponemal as well as rabbit host testicular tissue antigens. The majority (31 of 39 clones) of these anti-T. pallidum hybridomas, which produced monoclonal antibodies of mouse isotypes immunoglobulin G1 (IgG1), IgG2a, IgG2b, IgG3 or IgM, were directed specifically against T. pallidum and not other treponemal or rabbit antigens tested by radioimmunoassay. Four of these T. pallidum-specific hybridomas secreted monoclonal antibodies with greater binding affinity for 'aged' rather than freshly isolated intact T. pallidum cells, suggesting a possible specificity for 'unmasked' surface antigens of T. pallidum. Six anti-T. pallidum hybridomas produced complement-fixing monoclonal antibodies (IgG2a, IgG2b, or IgM) that were capable of immobilizing virulent treponemes in the T. pallidum immobilization (TPI) test; these may represent biologically active monoclonal antibodies against treponemal surface antigens. Three other hybridomas secreted monoclonal antibodies which bound to both T. pallidum and T. phagedenis biotype Reiter antigens, thus demonstrating a possible specificity for treponemal group antigens. Five hybridoma cell lines were also isolated which produced IgM monoclonal antibodies that cross-reacted with all treponemal and rabbit host testicular tissue antigens employed in the radioimmunoassays. This report describes the construction and characteristics of these hybridoma cell lines. The potential applications of the anti-T. pallidum monoclonal antibodies are discussed.

Original languageEnglish (US)
Pages (from-to)1076-1085
Number of pages10
JournalInfection and Immunity
Volume36
Issue number3
StatePublished - 1982

Fingerprint

Treponema pallidum
Hybridomas
Monoclonal Antibodies
Antigens
Globus Pallidus
Immunoglobulin M
Surface Antigens
Rabbits
Cell Line
Radioimmunoassay
Treponema Immobilization Test
Immunoglobulin Isotypes
Viral Tumor Antigens
Clone Cells
Immunoglobulin G
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols). / Robertson, S. M.; Kettman, J. R.; Miller, J. N.; Norgard, M. V.

In: Infection and Immunity, Vol. 36, No. 3, 1982, p. 1076-1085.

Research output: Contribution to journalArticle

Robertson, SM, Kettman, JR, Miller, JN & Norgard, MV 1982, 'Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols)', Infection and Immunity, vol. 36, no. 3, pp. 1076-1085.
Robertson, S. M. ; Kettman, J. R. ; Miller, J. N. ; Norgard, M. V. / Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols). In: Infection and Immunity. 1982 ; Vol. 36, No. 3. pp. 1076-1085.
@article{dbed5ec0831245d3b580d0b6be8f11b7,
title = "Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols)",
abstract = "Murine anti-Treponema pallidum (Nichols) lymphocyte hybridoma cell lines secreting monoclonal antibodies against a variety of treponemal antigens have been generated. Hybridomas isolated were of three major types: those that were directed specifically against T. pallidum antigens, those that were directed against treponemal group antigens (as evidenced by their cross-reactivity with T. phagedenis biotype Reiter antigens), and those that cross-reacted with both treponemal as well as rabbit host testicular tissue antigens. The majority (31 of 39 clones) of these anti-T. pallidum hybridomas, which produced monoclonal antibodies of mouse isotypes immunoglobulin G1 (IgG1), IgG2a, IgG2b, IgG3 or IgM, were directed specifically against T. pallidum and not other treponemal or rabbit antigens tested by radioimmunoassay. Four of these T. pallidum-specific hybridomas secreted monoclonal antibodies with greater binding affinity for 'aged' rather than freshly isolated intact T. pallidum cells, suggesting a possible specificity for 'unmasked' surface antigens of T. pallidum. Six anti-T. pallidum hybridomas produced complement-fixing monoclonal antibodies (IgG2a, IgG2b, or IgM) that were capable of immobilizing virulent treponemes in the T. pallidum immobilization (TPI) test; these may represent biologically active monoclonal antibodies against treponemal surface antigens. Three other hybridomas secreted monoclonal antibodies which bound to both T. pallidum and T. phagedenis biotype Reiter antigens, thus demonstrating a possible specificity for treponemal group antigens. Five hybridoma cell lines were also isolated which produced IgM monoclonal antibodies that cross-reacted with all treponemal and rabbit host testicular tissue antigens employed in the radioimmunoassays. This report describes the construction and characteristics of these hybridoma cell lines. The potential applications of the anti-T. pallidum monoclonal antibodies are discussed.",
author = "Robertson, {S. M.} and Kettman, {J. R.} and Miller, {J. N.} and Norgard, {M. V.}",
year = "1982",
language = "English (US)",
volume = "36",
pages = "1076--1085",
journal = "Infection and Immunity",
issn = "0019-9567",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Murine monoclonal antibodies specific for virulent Treponema pallidum (Nichols)

AU - Robertson, S. M.

AU - Kettman, J. R.

AU - Miller, J. N.

AU - Norgard, M. V.

PY - 1982

Y1 - 1982

N2 - Murine anti-Treponema pallidum (Nichols) lymphocyte hybridoma cell lines secreting monoclonal antibodies against a variety of treponemal antigens have been generated. Hybridomas isolated were of three major types: those that were directed specifically against T. pallidum antigens, those that were directed against treponemal group antigens (as evidenced by their cross-reactivity with T. phagedenis biotype Reiter antigens), and those that cross-reacted with both treponemal as well as rabbit host testicular tissue antigens. The majority (31 of 39 clones) of these anti-T. pallidum hybridomas, which produced monoclonal antibodies of mouse isotypes immunoglobulin G1 (IgG1), IgG2a, IgG2b, IgG3 or IgM, were directed specifically against T. pallidum and not other treponemal or rabbit antigens tested by radioimmunoassay. Four of these T. pallidum-specific hybridomas secreted monoclonal antibodies with greater binding affinity for 'aged' rather than freshly isolated intact T. pallidum cells, suggesting a possible specificity for 'unmasked' surface antigens of T. pallidum. Six anti-T. pallidum hybridomas produced complement-fixing monoclonal antibodies (IgG2a, IgG2b, or IgM) that were capable of immobilizing virulent treponemes in the T. pallidum immobilization (TPI) test; these may represent biologically active monoclonal antibodies against treponemal surface antigens. Three other hybridomas secreted monoclonal antibodies which bound to both T. pallidum and T. phagedenis biotype Reiter antigens, thus demonstrating a possible specificity for treponemal group antigens. Five hybridoma cell lines were also isolated which produced IgM monoclonal antibodies that cross-reacted with all treponemal and rabbit host testicular tissue antigens employed in the radioimmunoassays. This report describes the construction and characteristics of these hybridoma cell lines. The potential applications of the anti-T. pallidum monoclonal antibodies are discussed.

AB - Murine anti-Treponema pallidum (Nichols) lymphocyte hybridoma cell lines secreting monoclonal antibodies against a variety of treponemal antigens have been generated. Hybridomas isolated were of three major types: those that were directed specifically against T. pallidum antigens, those that were directed against treponemal group antigens (as evidenced by their cross-reactivity with T. phagedenis biotype Reiter antigens), and those that cross-reacted with both treponemal as well as rabbit host testicular tissue antigens. The majority (31 of 39 clones) of these anti-T. pallidum hybridomas, which produced monoclonal antibodies of mouse isotypes immunoglobulin G1 (IgG1), IgG2a, IgG2b, IgG3 or IgM, were directed specifically against T. pallidum and not other treponemal or rabbit antigens tested by radioimmunoassay. Four of these T. pallidum-specific hybridomas secreted monoclonal antibodies with greater binding affinity for 'aged' rather than freshly isolated intact T. pallidum cells, suggesting a possible specificity for 'unmasked' surface antigens of T. pallidum. Six anti-T. pallidum hybridomas produced complement-fixing monoclonal antibodies (IgG2a, IgG2b, or IgM) that were capable of immobilizing virulent treponemes in the T. pallidum immobilization (TPI) test; these may represent biologically active monoclonal antibodies against treponemal surface antigens. Three other hybridomas secreted monoclonal antibodies which bound to both T. pallidum and T. phagedenis biotype Reiter antigens, thus demonstrating a possible specificity for treponemal group antigens. Five hybridoma cell lines were also isolated which produced IgM monoclonal antibodies that cross-reacted with all treponemal and rabbit host testicular tissue antigens employed in the radioimmunoassays. This report describes the construction and characteristics of these hybridoma cell lines. The potential applications of the anti-T. pallidum monoclonal antibodies are discussed.

UR - http://www.scopus.com/inward/record.url?scp=0020053227&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020053227&partnerID=8YFLogxK

M3 - Article

VL - 36

SP - 1076

EP - 1085

JO - Infection and Immunity

JF - Infection and Immunity

SN - 0019-9567

IS - 3

ER -