Musashi1 Impacts Radio-Resistance in Glioblastoma by Controlling DNA-Protein Kinase Catalytic Subunit

Patricia Rosa de Araujo, Aparna Gorthi, Acarizia E. da Silva, Sonal S. Tonapi, Dat T. Vo, Suzanne C. Burns, Mei Qiao, Philip J. Uren, Zhi Min Yuan, Alexander J.R. Bishop, Luiz O.F. Penalva

Research output: Contribution to journalArticle

16 Scopus citations

Abstract

The conserved RNA-binding protein Musashi1 (MSI1) has been characterized as a stem cell marker, controlling the balance between self-renewal and differentiation and as a key oncogenic factor in numerous solid tumors, including glioblastoma. To explore the potential use of MSI1 targeting in therapy, we studied MSI1 in the context of radiation sensitivity. Knockdown of MSI1 led to a decrease in cell survival and an increase in DNA damage compared to control in cells treated with ionizing radiation. We subsequently examined mechanisms of double-strand break repair and found that loss of MSI1 reduces the frequency of nonhomologous end-joining. This phenomenon could be attributed to the decreased expression of DNA–protein kinase catalytic subunit, which we have previously identified as a target of MSI1. Collectively, our results suggest a role for MSI1 in double-strand break repair and that its inhibition may enhance the effect of radiotherapy.

Original languageEnglish (US)
Pages (from-to)2271-2278
Number of pages8
JournalAmerican Journal of Pathology
Volume186
Issue number9
DOIs
StatePublished - Sep 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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    de Araujo, P. R., Gorthi, A., da Silva, A. E., Tonapi, S. S., Vo, D. T., Burns, S. C., Qiao, M., Uren, P. J., Yuan, Z. M., Bishop, A. J. R., & Penalva, L. O. F. (2016). Musashi1 Impacts Radio-Resistance in Glioblastoma by Controlling DNA-Protein Kinase Catalytic Subunit. American Journal of Pathology, 186(9), 2271-2278. https://doi.org/10.1016/j.ajpath.2016.05.020