Muscarinic control of MIN6 pancreatic β cells is enhanced by impaired amino acid signaling

Marcy L. Guerra, Eric M. Wauson, Kathleen McGlynn, Melanie H. Cobb

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We have shown recently that the class C G protein-coupled receptor T1R1/T1R3 taste receptor complex is an early amino acid sensor in MIN6 pancreatic β cells. Amino acids are unable to activate ERK1/2 in β cells in which T1R3 has been depleted. The muscarinic receptor agonist carbachol activated ERK1/2 better in T1R3-depleted cells than in control cells. Ligands that activate certain G protein-coupled receptors in pancreatic β cells potentiate glucose-stimulated insulin secretion. Among these is the M3 muscarinic acetylcholine receptor, the major muscarinic receptor in β cells. We found that expression of M3 receptors increased in T1R3-depleted MIN6 cells and that calcium responses were altered. To determine whether these changes were related to impaired amino acid signaling, we compared responses in cells exposed to reduced amino acid concentrations. M3 receptor expression was increased, and some, but not all, changes in calcium signaling were mimicked. These findings suggest that M3 acetylcholine receptors are increased in β cells as a mechanism to compensate for amino acid deficiency.

Original languageEnglish (US)
Pages (from-to)14370-14379
Number of pages10
JournalJournal of Biological Chemistry
Volume289
Issue number20
DOIs
StatePublished - May 16 2014

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Cholinergic Agents
Amino Acids
Muscarinic Receptors
G-Protein-Coupled Receptors
Calcium
Carbachol
Cholinergic Receptors
Muscarinic Agonists
Insulin
Ligands
Calcium Signaling
Glucose
Sensors

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Muscarinic control of MIN6 pancreatic β cells is enhanced by impaired amino acid signaling. / Guerra, Marcy L.; Wauson, Eric M.; McGlynn, Kathleen; Cobb, Melanie H.

In: Journal of Biological Chemistry, Vol. 289, No. 20, 16.05.2014, p. 14370-14379.

Research output: Contribution to journalArticle

Guerra, Marcy L. ; Wauson, Eric M. ; McGlynn, Kathleen ; Cobb, Melanie H. / Muscarinic control of MIN6 pancreatic β cells is enhanced by impaired amino acid signaling. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 20. pp. 14370-14379.
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