Muscle-specific RNA processing continues in the absence of myogenin expression

Osamu Saitoh, Eric N. Olson, Muthu Periasamy

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

During muscle development, muscle-specific gene expression is achieved by a complex set of transcriptional and post-transcriptional mechanisms. We examined the requirement of new protein synthesis and myogenin expression for muscle-specific tropomyosin expression during myogenesis of the rat muscle cell line L6E9. The results show that new protein synthesis is required for both muscle gene transcription and muscle-specific alternate RNA processing. However, once initiated, the synthesis of muscle tropomyosin mRNA continued in the presence of cycloheximide, while the expression of muscle actin, myosin heavy chain, and myogenin mRNA was abolished. These results suggest that muscle-specific processing of tropomyosin transcripts can continue to occur in the absence of myogenin expression unlike the expression of muscle actin and myosin heavy chain mRNAs. However, the transfection of myogenin cDNA into nonmuscle cells induced muscle-specific RNA processing of tropomyosin, suggesting indirect involvement of myogenic factors in the initiation of muscle-specific RNA processing pathways.

Original languageEnglish (US)
Pages (from-to)19381-19384
Number of pages4
JournalJournal of Biological Chemistry
Volume265
Issue number32
StatePublished - Nov 15 1990

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Myogenin
Muscle
RNA
Muscles
Tropomyosin
Processing
Myosin Heavy Chains
Muscle Development
Muscle Cells
Messenger RNA
Actins
Peptide Initiation Factors
Cycloheximide
Transfection
Proteins
Transcription
Complementary DNA
Gene expression
Gene Expression
Rats

ASJC Scopus subject areas

  • Biochemistry

Cite this

Muscle-specific RNA processing continues in the absence of myogenin expression. / Saitoh, Osamu; Olson, Eric N.; Periasamy, Muthu.

In: Journal of Biological Chemistry, Vol. 265, No. 32, 15.11.1990, p. 19381-19384.

Research output: Contribution to journalArticle

Saitoh, Osamu ; Olson, Eric N. ; Periasamy, Muthu. / Muscle-specific RNA processing continues in the absence of myogenin expression. In: Journal of Biological Chemistry. 1990 ; Vol. 265, No. 32. pp. 19381-19384.
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