Mutation of 3' splice sites in two different class I genes results in different usage of cryptic splice sites

M. L. Hedley, J. Forman, P. W. Tucker

Research output: Contribution to journalArticle

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Abstract

To determine the pattern of alternative splicing at the 5' end of class I genes, the 3' splice sites bordering exon 2 of the H-2D(d) and H-2K(d) genes were mutated from AG to GG (H-2D(d)) or CG (H-2K(d)). The mutant genes were transfected into L cells, and RNA from clones expressing these Ag was used for analysis by RNase and S1 nuclease mapping techniques. The first intervening sequence of both class I genes contains several potential 3' splice acceptor sites. However, a clear preference for only one site was detected in each of the H-2D(d) and H-2K(d) mRNA. Examination of the endogenous H-D(d) and H-2K(d) class I transcripts in normal murine tissues and in tumors demonstrated that the alternatively spliced mRNAs were produced, but at a low frequency. Infection of transfected L cells or tumor lines with vesicular stomatitis virus altered the level of differentially spliced message in these cells.

Original languageEnglish (US)
Pages (from-to)1018-1025
Number of pages8
JournalJournal of Immunology
Volume143
Issue number3
StatePublished - Jan 1 1989

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MHC Class I Genes
RNA Splice Sites
Mutation
Messenger RNA
Vesicular Stomatitis
Alternative Splicing
Tumor Cell Line
Introns
Genes
Exons
Clone Cells
RNA
Viruses
Infection
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Mutation of 3' splice sites in two different class I genes results in different usage of cryptic splice sites. / Hedley, M. L.; Forman, J.; Tucker, P. W.

In: Journal of Immunology, Vol. 143, No. 3, 01.01.1989, p. 1018-1025.

Research output: Contribution to journalArticle

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