Mutation of 3' splice sites in two different class I genes results in different usage of cryptic splice sites

M. L. Hedley, J. Forman, P. W. Tucker

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

To determine the pattern of alternative splicing at the 5' end of class I genes, the 3' splice sites bordering exon 2 of the H-2D(d) and H-2K(d) genes were mutated from AG to GG (H-2D(d)) or CG (H-2K(d)). The mutant genes were transfected into L cells, and RNA from clones expressing these Ag was used for analysis by RNase and S1 nuclease mapping techniques. The first intervening sequence of both class I genes contains several potential 3' splice acceptor sites. However, a clear preference for only one site was detected in each of the H-2D(d) and H-2K(d) mRNA. Examination of the endogenous H-D(d) and H-2K(d) class I transcripts in normal murine tissues and in tumors demonstrated that the alternatively spliced mRNAs were produced, but at a low frequency. Infection of transfected L cells or tumor lines with vesicular stomatitis virus altered the level of differentially spliced message in these cells.

Original languageEnglish (US)
Pages (from-to)1018-1025
Number of pages8
JournalJournal of Immunology
Volume143
Issue number3
StatePublished - Jan 1 1989

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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