Abstract
To determine the pattern of alternative splicing at the 5' end of class I genes, the 3' splice sites bordering exon 2 of the H-2D(d) and H-2K(d) genes were mutated from AG to GG (H-2D(d)) or CG (H-2K(d)). The mutant genes were transfected into L cells, and RNA from clones expressing these Ag was used for analysis by RNase and S1 nuclease mapping techniques. The first intervening sequence of both class I genes contains several potential 3' splice acceptor sites. However, a clear preference for only one site was detected in each of the H-2D(d) and H-2K(d) mRNA. Examination of the endogenous H-D(d) and H-2K(d) class I transcripts in normal murine tissues and in tumors demonstrated that the alternatively spliced mRNAs were produced, but at a low frequency. Infection of transfected L cells or tumor lines with vesicular stomatitis virus altered the level of differentially spliced message in these cells.
Original language | English (US) |
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Pages (from-to) | 1018-1025 |
Number of pages | 8 |
Journal | Journal of Immunology |
Volume | 143 |
Issue number | 3 |
State | Published - 1989 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology