Mutations in a conserved domain of E. coli MscS to the most conserved superfamily residue leads to kinetic changes

Hannah R. Malcolm, Paul Blount

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In Escherichia coli (E. coli) the mechanosensitive channel of small conductance, MscS, gates in response to membrane tension created from acute external hypoosmotic shock, thus rescuing the bacterium from cell lysis. E. coli MscS is the most well studied member of the MscS superfamily of channels, whose members are found throughout the bacterial and plant kingdoms. Homology to the pore lining helix and upper vestibule domain of E. coli MscS is required for inclusion into the superfamily. Although highly conserved, in the second half of the pore lining helix (TM3B), E. coli MscS has five residues significantly different from other members of the superfamily. In superfamilies such as this, it remains unclear why variations within such a homologous region occur: is it tolerance of alternate residues, or does it define functional variance within the superfamily? Point mutations (S114I/T, L118F, A120S, L123F, F127E/K/T) and patch clamp electrophysiology were used to study the effect of changing these residues in E. coli MscS on sensitivity and gating. The data indicate that variation at these locations do not consistently lead to wildtype channel phenotypes, nor do they define large changes in mechanosensation, but often appear to effect changes in the E. coli MscS channel gating kinetics.

Original languageEnglish (US)
Article numbere0136756
JournalPLoS One
Volume10
Issue number9
DOIs
StatePublished - Sep 4 2015

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Escherichia coli
mutation
kinetics
Mutation
Kinetics
Linings
Electrophysiology
electrophysiology
Clamping devices
point mutation
Point Mutation
Shock
Bacteria
Membranes
Phenotype
phenotype
bacteria
cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Mutations in a conserved domain of E. coli MscS to the most conserved superfamily residue leads to kinetic changes. / Malcolm, Hannah R.; Blount, Paul.

In: PLoS One, Vol. 10, No. 9, e0136756, 04.09.2015.

Research output: Contribution to journalArticle

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