Mutations in human dynamin block an intermediate stage in coated vesicle formation

Alexander M. Van Der Bliek, Thomas E. Redelmeier, Hanna Damke, Ellen J. Tisdale, Elliot M. Meyerowitz, Sandra L. Schmid

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Abstract

The role of human dynamin in receptor-mediated endocytosis was investigated by transient expression of GTP-binding domain mutants in mammalian cells. Using assays which detect intermediates in coated vesicle formation, the dynamin mutants were found to block endocytosis at a stage after the initiation of coat assembly and preceding the sequestration of ligands into deeply invaginated coated pits. Membrane transport from the ER to the Golgi complex was unaffected indicating that dynamin mutants specifically block early events in endocytosis. These results demonstrate that mutations in the GTP-binding domain of dynamin block Tfn-endocytosis in mammalian cells and suggest that a functional dynamin GTPase is required for receptor-mediated endocytosis via clathrincoated pits.

Original languageEnglish (US)
Pages (from-to)553-563
Number of pages11
JournalJournal of Cell Biology
Volume122
Issue number3
Publication statusPublished - Aug 1993

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ASJC Scopus subject areas

  • Cell Biology

Cite this

Van Der Bliek, A. M., Redelmeier, T. E., Damke, H., Tisdale, E. J., Meyerowitz, E. M., & Schmid, S. L. (1993). Mutations in human dynamin block an intermediate stage in coated vesicle formation. Journal of Cell Biology, 122(3), 553-563.