Mutations in the midway gene disrupt a Drosophila acyl coenzyme A

Diacylglycerol acyltransferase

Michael Buszczak, Xiaohui Lu, William A. Segraves, Ta Yuan Chang, Lynn Cooley

Research output: Contribution to journalArticle

61 Citations (Scopus)

Abstract

During Drosophila oogenesis, defective or unwanted egg chambers are eliminated during mid-oogenesis by programmed cell death. In addition, final cytoplasm transport from nurse cells to the oocyte depends upon apoptosis of the nurse cells. To study the regulation of germline apoptosis, we analyzed the midway mutant, in which egg chambers undergo premature nurse cell death and degeneration. The midway gene encodes a protein similar to mammalian acyl coenzyme A: diacylglycerol acyltransferase (DGAT), which converts diacylglycerol (DAG) into triacylglycerol (TAG). midway mutant egg chambers contain severely reduced levels of neutral lipids in the germline. Expression of midway in insect cells results in high levels of DGAT activity in vitro. These results show that midway encodes a functional DGAT and that changes in acyglycerol lipid metabolism disrupt normal egg chamber development in Drosophila.

Original languageEnglish (US)
Pages (from-to)1511-1518
Number of pages8
JournalGenetics
Volume160
Issue number4
StatePublished - 2002

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Diacylglycerol O-Acyltransferase
Acyl Coenzyme A
Drosophila
Ovum
Oogenesis
Mutation
Nurses
Genes
Cell Death
Apoptosis
Diglycerides
Lipid Metabolism
Oocytes
Insects
Cytoplasm
Triglycerides
Lipids
Proteins

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Buszczak, M., Lu, X., Segraves, W. A., Chang, T. Y., & Cooley, L. (2002). Mutations in the midway gene disrupt a Drosophila acyl coenzyme A: Diacylglycerol acyltransferase. Genetics, 160(4), 1511-1518.

Mutations in the midway gene disrupt a Drosophila acyl coenzyme A : Diacylglycerol acyltransferase. / Buszczak, Michael; Lu, Xiaohui; Segraves, William A.; Chang, Ta Yuan; Cooley, Lynn.

In: Genetics, Vol. 160, No. 4, 2002, p. 1511-1518.

Research output: Contribution to journalArticle

Buszczak, M, Lu, X, Segraves, WA, Chang, TY & Cooley, L 2002, 'Mutations in the midway gene disrupt a Drosophila acyl coenzyme A: Diacylglycerol acyltransferase', Genetics, vol. 160, no. 4, pp. 1511-1518.
Buszczak, Michael ; Lu, Xiaohui ; Segraves, William A. ; Chang, Ta Yuan ; Cooley, Lynn. / Mutations in the midway gene disrupt a Drosophila acyl coenzyme A : Diacylglycerol acyltransferase. In: Genetics. 2002 ; Vol. 160, No. 4. pp. 1511-1518.
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