Mutations in the p53 gene in primary human breast cancers

R. J. Osborne, G. R. Merlo, T. Mitsudomi, T. Venesio, D. S. Liscia, A. P M Cappa, I. Chiba, T. Takahashi, M. M. Nau, R. Callahan, J. D. Minna

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Abstract

Twenty-six primary breast tumors were examined for mutations in the p53 tumor suppressor gene by an RNase protection assay and nucleotide sequence analysis of PCR-amplified p53 complementary DNAs. Each method detected p53 mutations in the same three tumors (12%). One tumor contained two mutations in the same allele. Single strand conformation polymorphism analysis of genomic DNA and complementary DNA proved more sensitive in the detection of mutations. Combining this technique with the other two a total of 12 mutations in the p53 gene were demonstrated in 11 tumors (46%), and a polymorphism at codon 213 was detected in another tumor. Loss of heterozygosity on chromosome 17p was detected by Southern blot analysis in 30% of the tumor DNAs. Not all of the tumors containing a point mutation in p53 also had loss of heterozygosity of the remaining allele, suggesting that loss of heterozygosity may represent a later event.

Original languageEnglish (US)
Pages (from-to)6194-6198
Number of pages5
JournalCancer Research
Volume51
Issue number22
StatePublished - Nov 15 1991

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p53 Genes
Breast Neoplasms
Mutation
Loss of Heterozygosity
Neoplasms
Complementary DNA
Alleles
DNA
Ribonucleases
Southern Blotting
Tumor Suppressor Genes
Point Mutation
Codon
Sequence Analysis
Chromosomes
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Osborne, R. J., Merlo, G. R., Mitsudomi, T., Venesio, T., Liscia, D. S., Cappa, A. P. M., ... Minna, J. D. (1991). Mutations in the p53 gene in primary human breast cancers. Cancer Research, 51(22), 6194-6198.

Mutations in the p53 gene in primary human breast cancers. / Osborne, R. J.; Merlo, G. R.; Mitsudomi, T.; Venesio, T.; Liscia, D. S.; Cappa, A. P M; Chiba, I.; Takahashi, T.; Nau, M. M.; Callahan, R.; Minna, J. D.

In: Cancer Research, Vol. 51, No. 22, 15.11.1991, p. 6194-6198.

Research output: Contribution to journalArticle

Osborne, RJ, Merlo, GR, Mitsudomi, T, Venesio, T, Liscia, DS, Cappa, APM, Chiba, I, Takahashi, T, Nau, MM, Callahan, R & Minna, JD 1991, 'Mutations in the p53 gene in primary human breast cancers', Cancer Research, vol. 51, no. 22, pp. 6194-6198.
Osborne RJ, Merlo GR, Mitsudomi T, Venesio T, Liscia DS, Cappa APM et al. Mutations in the p53 gene in primary human breast cancers. Cancer Research. 1991 Nov 15;51(22):6194-6198.
Osborne, R. J. ; Merlo, G. R. ; Mitsudomi, T. ; Venesio, T. ; Liscia, D. S. ; Cappa, A. P M ; Chiba, I. ; Takahashi, T. ; Nau, M. M. ; Callahan, R. ; Minna, J. D. / Mutations in the p53 gene in primary human breast cancers. In: Cancer Research. 1991 ; Vol. 51, No. 22. pp. 6194-6198.
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