Mutations of ras genes distinguish a subset of non-small-cell lung cancer cell lines from small-cell lung cancer cell lines

Tetsuya Mitsudomi, Jean Viallet, James L. Mulshine, R. Ilona Linnoila, John D. Minna, Adi F. Gazdar

Research output: Contribution to journalArticle

283 Citations (Scopus)

Abstract

We screened a panel of 103 human lung cancer cell lines for the presence of point mutations at codons 12, 13 or 61 of the human K-, H- and N-ras genes, using restriction fragment length polymorphisms (RFLP), created through mismatched primers during polymerase chain reaction (PCR) of genomic DNA. We found ras mutations in 22/61 (36%) non-small-cell lung cancer (NSCLC) cell lines, predominantly in K-ras codon 12. Identical mutations were present in uncultured tumor materials corresponding to 11 cell lines containing mutated ras genes. ras mutations were found not only in adenocarcinoma cell lines (9/32, 28%), but also in cell lines derived from other type of NSCLC (13/29, 45%). In contrast, none of 37 small-cell lung cancer (SCLC) cell lines and five extrapulmonary small-cell cancer cell lines had ras mutations. ras mutations were not correlated with sex of the patients, tumor extent, prior therapy status or in vitro culture time. G to T or A to T transversions were the most common base substitutions, occurring in codons 12 and 61 respectively. We conclude that ras mutations play a role in the pathogenesis of a subset of NSCLC but are not involved in SCLC.

Original languageEnglish (US)
Pages (from-to)1353-1362
Number of pages10
JournalOncogene
Volume6
Issue number8
StatePublished - Aug 1991

Fingerprint

ras Genes
Small Cell Lung Carcinoma
Non-Small Cell Lung Carcinoma
Cell Line
Mutation
Codon
Neoplasms
Point Mutation
Restriction Fragment Length Polymorphisms
Lung Neoplasms
Adenocarcinoma
Polymerase Chain Reaction
DNA

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Mitsudomi, T., Viallet, J., Mulshine, J. L., Linnoila, R. I., Minna, J. D., & Gazdar, A. F. (1991). Mutations of ras genes distinguish a subset of non-small-cell lung cancer cell lines from small-cell lung cancer cell lines. Oncogene, 6(8), 1353-1362.

Mutations of ras genes distinguish a subset of non-small-cell lung cancer cell lines from small-cell lung cancer cell lines. / Mitsudomi, Tetsuya; Viallet, Jean; Mulshine, James L.; Linnoila, R. Ilona; Minna, John D.; Gazdar, Adi F.

In: Oncogene, Vol. 6, No. 8, 08.1991, p. 1353-1362.

Research output: Contribution to journalArticle

Mitsudomi, T, Viallet, J, Mulshine, JL, Linnoila, RI, Minna, JD & Gazdar, AF 1991, 'Mutations of ras genes distinguish a subset of non-small-cell lung cancer cell lines from small-cell lung cancer cell lines', Oncogene, vol. 6, no. 8, pp. 1353-1362.
Mitsudomi, Tetsuya ; Viallet, Jean ; Mulshine, James L. ; Linnoila, R. Ilona ; Minna, John D. ; Gazdar, Adi F. / Mutations of ras genes distinguish a subset of non-small-cell lung cancer cell lines from small-cell lung cancer cell lines. In: Oncogene. 1991 ; Vol. 6, No. 8. pp. 1353-1362.
@article{296c59dde5f54f9ebe3d7166a35d15d7,
title = "Mutations of ras genes distinguish a subset of non-small-cell lung cancer cell lines from small-cell lung cancer cell lines",
abstract = "We screened a panel of 103 human lung cancer cell lines for the presence of point mutations at codons 12, 13 or 61 of the human K-, H- and N-ras genes, using restriction fragment length polymorphisms (RFLP), created through mismatched primers during polymerase chain reaction (PCR) of genomic DNA. We found ras mutations in 22/61 (36{\%}) non-small-cell lung cancer (NSCLC) cell lines, predominantly in K-ras codon 12. Identical mutations were present in uncultured tumor materials corresponding to 11 cell lines containing mutated ras genes. ras mutations were found not only in adenocarcinoma cell lines (9/32, 28{\%}), but also in cell lines derived from other type of NSCLC (13/29, 45{\%}). In contrast, none of 37 small-cell lung cancer (SCLC) cell lines and five extrapulmonary small-cell cancer cell lines had ras mutations. ras mutations were not correlated with sex of the patients, tumor extent, prior therapy status or in vitro culture time. G to T or A to T transversions were the most common base substitutions, occurring in codons 12 and 61 respectively. We conclude that ras mutations play a role in the pathogenesis of a subset of NSCLC but are not involved in SCLC.",
author = "Tetsuya Mitsudomi and Jean Viallet and Mulshine, {James L.} and Linnoila, {R. Ilona} and Minna, {John D.} and Gazdar, {Adi F.}",
year = "1991",
month = "8",
language = "English (US)",
volume = "6",
pages = "1353--1362",
journal = "Oncogene",
issn = "0950-9232",
publisher = "Nature Publishing Group",
number = "8",

}

TY - JOUR

T1 - Mutations of ras genes distinguish a subset of non-small-cell lung cancer cell lines from small-cell lung cancer cell lines

AU - Mitsudomi, Tetsuya

AU - Viallet, Jean

AU - Mulshine, James L.

AU - Linnoila, R. Ilona

AU - Minna, John D.

AU - Gazdar, Adi F.

PY - 1991/8

Y1 - 1991/8

N2 - We screened a panel of 103 human lung cancer cell lines for the presence of point mutations at codons 12, 13 or 61 of the human K-, H- and N-ras genes, using restriction fragment length polymorphisms (RFLP), created through mismatched primers during polymerase chain reaction (PCR) of genomic DNA. We found ras mutations in 22/61 (36%) non-small-cell lung cancer (NSCLC) cell lines, predominantly in K-ras codon 12. Identical mutations were present in uncultured tumor materials corresponding to 11 cell lines containing mutated ras genes. ras mutations were found not only in adenocarcinoma cell lines (9/32, 28%), but also in cell lines derived from other type of NSCLC (13/29, 45%). In contrast, none of 37 small-cell lung cancer (SCLC) cell lines and five extrapulmonary small-cell cancer cell lines had ras mutations. ras mutations were not correlated with sex of the patients, tumor extent, prior therapy status or in vitro culture time. G to T or A to T transversions were the most common base substitutions, occurring in codons 12 and 61 respectively. We conclude that ras mutations play a role in the pathogenesis of a subset of NSCLC but are not involved in SCLC.

AB - We screened a panel of 103 human lung cancer cell lines for the presence of point mutations at codons 12, 13 or 61 of the human K-, H- and N-ras genes, using restriction fragment length polymorphisms (RFLP), created through mismatched primers during polymerase chain reaction (PCR) of genomic DNA. We found ras mutations in 22/61 (36%) non-small-cell lung cancer (NSCLC) cell lines, predominantly in K-ras codon 12. Identical mutations were present in uncultured tumor materials corresponding to 11 cell lines containing mutated ras genes. ras mutations were found not only in adenocarcinoma cell lines (9/32, 28%), but also in cell lines derived from other type of NSCLC (13/29, 45%). In contrast, none of 37 small-cell lung cancer (SCLC) cell lines and five extrapulmonary small-cell cancer cell lines had ras mutations. ras mutations were not correlated with sex of the patients, tumor extent, prior therapy status or in vitro culture time. G to T or A to T transversions were the most common base substitutions, occurring in codons 12 and 61 respectively. We conclude that ras mutations play a role in the pathogenesis of a subset of NSCLC but are not involved in SCLC.

UR - http://www.scopus.com/inward/record.url?scp=0025785811&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025785811&partnerID=8YFLogxK

M3 - Article

C2 - 1679529

AN - SCOPUS:0025785811

VL - 6

SP - 1353

EP - 1362

JO - Oncogene

JF - Oncogene

SN - 0950-9232

IS - 8

ER -