Mutual enhancement of IL-2 and IL-7 on DNA vaccine immunogenicity mainly involves regulations on their receptor expression and receptor-expressing lymphocyte generation

Yonghong Zhang, Shuang Liang, Xiujin Li, Liyue Wang, Jianlou Zhang, Jian Xu, Shanshan Huo, Xuebin Cao, Zhenyu Zhong, Fei Zhong

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Our previous study showed that IL-2 and IL-7 could mutually enhance the immunogenicity of canine parvovirus VP2 DNA vaccine, although the underlying mechanism remained unknown. Here, we used the OVA gene as a DNA vaccine in a mouse model to test their enhancement on DNA vaccine immunogenicity and to explore the molecular mechanism. Results showed that both IL-2 and IL-7 genes significantly increased the immunogenicity of OVA DNA vaccine in mice. Co-administration of IL-2 and IL-7 genes with OVA DNA significantly increased OVA-specific antibody titers, T cell proliferation and IFN-γ production compared with IL-2 or IL-7 alone, confirming that IL-2 and IL-7 mutually enhanced DNA vaccine immunogenicity. Mechanistically, we have shown that IL-2 significantly stimulated generation of IL-7 receptor-expressing lymphocytes, and that IL-7 significantly induced IL-2 receptor expression. These results contribute to an explanation of the mechanism of the mutual effects of IL-2 and IL-7 on enhancing DNA vaccine immunogenicity and provided a basis for further investigation on their mutual effects on adjuvant activity and immune regulation.

Original languageEnglish (US)
Pages (from-to)3480-3487
Number of pages8
JournalVaccine
Volume33
Issue number30
DOIs
StatePublished - Jul 9 2015
Externally publishedYes

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interleukin-7
Interleukin-7
DNA Vaccines
recombinant vaccines
interleukin-2
Interleukin-2
lymphocytes
immune response
Lymphocytes
receptors
Interleukin-7 Receptors
Canine Parvovirus
Genes
Interleukin-2 Receptors
Carnivore protoparvovirus 1
genes
Vaccine Immunogenicity
adjuvants
cell proliferation
Cell Proliferation

Keywords

  • DNA vaccine
  • Interleukin-2
  • Interleukin-7
  • Mutual effects

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Mutual enhancement of IL-2 and IL-7 on DNA vaccine immunogenicity mainly involves regulations on their receptor expression and receptor-expressing lymphocyte generation. / Zhang, Yonghong; Liang, Shuang; Li, Xiujin; Wang, Liyue; Zhang, Jianlou; Xu, Jian; Huo, Shanshan; Cao, Xuebin; Zhong, Zhenyu; Zhong, Fei.

In: Vaccine, Vol. 33, No. 30, 09.07.2015, p. 3480-3487.

Research output: Contribution to journalArticle

Zhang, Yonghong ; Liang, Shuang ; Li, Xiujin ; Wang, Liyue ; Zhang, Jianlou ; Xu, Jian ; Huo, Shanshan ; Cao, Xuebin ; Zhong, Zhenyu ; Zhong, Fei. / Mutual enhancement of IL-2 and IL-7 on DNA vaccine immunogenicity mainly involves regulations on their receptor expression and receptor-expressing lymphocyte generation. In: Vaccine. 2015 ; Vol. 33, No. 30. pp. 3480-3487.
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