MYCN silencing induces differentiation and apoptosis in human neuroblastoma cells

Jung Hee Kang, Piotr G. Rychahou, Titilope A. Ishola, Jingbo Qiao, B. Mark Evers, Dai H. Chung

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

MYCN amplification strongly correlates with unfavorable outcomes in patients with neuroblastoma. The aim of this study was to investigate the role of MYCN in neuroblastoma cell differentiation and apoptosis. We used the technique of RNA interference to inhibit MYCN gene expression in neuroblastoma cells with variable expression of MYCN. Our results showed that inhibition of MYCN gene expression in MYCN amplified cells induced apoptosis and suppressed cell growth; neuronal differentiation also occurred after MYCN gene silencing. Moreover, N-myc downregulation was associated with decreased Bcl-xL protein levels and caspase-3 activation. These data show that small interfering RNA directed to MYCN, which plays a crucial role in neuroblastoma cell survival, may provide a potential novel therapeutic option for aggressive neuroblastomas.

Original languageEnglish (US)
Pages (from-to)192-197
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume351
Issue number1
DOIs
StatePublished - Dec 8 2006
Externally publishedYes

Fingerprint

Neuroblastoma
Gene expression
Apoptosis
Cell growth
Caspase 3
Small Interfering RNA
Amplification
Genes
Chemical activation
Cells
RNA
Gene Expression
Gene Silencing
RNA Interference
Proteins
Cell Differentiation
Cell Survival
Down-Regulation
Growth
Therapeutics

Keywords

  • Apoptosis
  • Differentiation
  • MYCN/N-myc
  • Neuroblastoma
  • RNAi/siRNA

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

MYCN silencing induces differentiation and apoptosis in human neuroblastoma cells. / Kang, Jung Hee; Rychahou, Piotr G.; Ishola, Titilope A.; Qiao, Jingbo; Evers, B. Mark; Chung, Dai H.

In: Biochemical and Biophysical Research Communications, Vol. 351, No. 1, 08.12.2006, p. 192-197.

Research output: Contribution to journalArticle

Kang, Jung Hee ; Rychahou, Piotr G. ; Ishola, Titilope A. ; Qiao, Jingbo ; Evers, B. Mark ; Chung, Dai H. / MYCN silencing induces differentiation and apoptosis in human neuroblastoma cells. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 351, No. 1. pp. 192-197.
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