Mycoplasma pneumoniae induces chronic respiratory infection, airway hyperreactivity, and pulmonary inflammation

A murine model of infection-associated chronic reactive airway disease

Robert D. Hardy, Hasan S. Jafri, Kurt Olsen, Jeanine Hatfield, Janie Iglehart, Beverly B. Rogers, Padma Patel, Gail Cassell, George H. McCracken, Octavio Ramilo

Research output: Contribution to journalArticle

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Abstract

Because chronic Mycoplasma pneumoniae respiratory infection is hypothesized to play a role in asthma, the potential of M. pneumoniae to establish chronic respiratory infection with associated pulmonary disease was investigated in a murine model. BALB/c mice were intranasally inoculated once with M. pneumoniae and examined at 109, 150, 245, 368, and 530 days postinoculation. M. pneumoniae was detected in bronchoalveolar lavage fluid by culture or PCR in 70 and 22% of mice at 109 and 530 days postinoculation, respectively. Lung histopathology was normal up to 368 days postinoculation. At 530 days, however, 78% of the mice inoculated with M. pneumoniae demonstrated abnormal histopathology characterized by peribronchial and perivascular mononuclear infiltrates. A mean histopathologic score (HPS) at 530 days of 5.1 was significantly greater (P < 0.01) than that for controls (HPS score of 0). Serum anti-M. pneumoniae immunoglobulin G was detectable in all of the mice inoculated with M. pneumoniae and was inversely correlated with HPS (r = -0.95, P = 0.01) at 530 days postinoculation. Unrestrained whole-body plethysmography measurement of enhanced pause revealed significantly elevated airway methacholine reactivity in M. pneumoniae-inoculated mice compared with that in controls at 245 days (P = 0.03) and increased airway obstruction at 530 days (P = 0.01). Murine M. pneumoniae respiratory infection can lead to chronic pulmonary disease characterized by airway hyperre activity, airway obstruction, and histologic inflammation.

Original languageEnglish (US)
Pages (from-to)649-654
Number of pages6
JournalInfection and Immunity
Volume70
Issue number2
DOIs
StatePublished - 2002

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Mycoplasma pneumoniae
Respiratory Tract Infections
Pneumonia
Infection
Airway Obstruction
Lung Diseases
Whole Body Plethysmography
Methacholine Chloride
Bronchoalveolar Lavage Fluid
Chronic Disease
Asthma
Immunoglobulin G
Inflammation
Polymerase Chain Reaction
Lung

ASJC Scopus subject areas

  • Immunology

Cite this

Mycoplasma pneumoniae induces chronic respiratory infection, airway hyperreactivity, and pulmonary inflammation : A murine model of infection-associated chronic reactive airway disease. / Hardy, Robert D.; Jafri, Hasan S.; Olsen, Kurt; Hatfield, Jeanine; Iglehart, Janie; Rogers, Beverly B.; Patel, Padma; Cassell, Gail; McCracken, George H.; Ramilo, Octavio.

In: Infection and Immunity, Vol. 70, No. 2, 2002, p. 649-654.

Research output: Contribution to journalArticle

Hardy, Robert D. ; Jafri, Hasan S. ; Olsen, Kurt ; Hatfield, Jeanine ; Iglehart, Janie ; Rogers, Beverly B. ; Patel, Padma ; Cassell, Gail ; McCracken, George H. ; Ramilo, Octavio. / Mycoplasma pneumoniae induces chronic respiratory infection, airway hyperreactivity, and pulmonary inflammation : A murine model of infection-associated chronic reactive airway disease. In: Infection and Immunity. 2002 ; Vol. 70, No. 2. pp. 649-654.
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