Mycotoxin zearalenone attenuates innate immune responses and suppresses nlrp3 inflammasome activation in lps-activated macrophages

Po Yen Lee, Ching Chih Liu, Shu Chi Wang, Kai Yin Chen, Tzu Chieh Lin, Po Len Liu, Chien Chih Chiu, I. Chen Chen, Yu Hung Lai, Wei Chung Cheng, Wei Ju Chung, Hsin Chih Yeh, Chi Han Huang, Chia Cheng Su, Shu Pin Huang, Chia Yang Li

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Zearalenone (ZEA) is a mycotoxin that has several adverse effects on most mammalian species. However, the effects of ZEA on macrophage-mediated innate immunity during infection have not been examined. In the present study, bacterial lipopolysaccharides (LPS) were used to induce the activation of macrophages and evaluate the effects of ZEA on the inflammatory responses and inflammation-associated signaling pathways. The experimental results indicated that ZEA suppressed LPS-activated inflammatory responses by macrophages including attenuating the production of proinflammatory mediators (nitric oxide (NO) and prostaglandin E2 (PGE2 )), decreased the secretion of proinflammatory cytokines (tumor necrosis factor (TNF)-α, interleukin (IL)-1β and IL-6), inhibited the activation of c-Jun amino-terminal kinase (JNK), p38 and nuclear factor-κB (NF-κB) signaling pathways, and repressed the nucleotide-binding and oligomerization domain (NOD)-, leucine-rich repeat (LRR)-and pyrin domain-containing protein 3 (NLRP3) inflammasome activation. These results indicated that mycotoxin ZEA attenuates macrophage-mediated innate immunity upon LPS stimulation, suggesting that the intake of mycotoxin ZEA-contaminated food might result in decreasing innate immunity, which has a higher risk of adverse effects during infection.

Original languageEnglish (US)
Article number593
JournalToxins
Volume13
Issue number9
DOIs
StatePublished - Sep 2021
Externally publishedYes

Keywords

  • Innate immunity
  • Macrophages
  • Mycotoxin
  • NLRP3 inflammasome
  • Zearalenone

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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