TY - JOUR
T1 - Myogenin, a factor regulating myogenesis, has a domain homologous to MyoD
AU - Wright, Woodring E.
AU - Sassoon, David A.
AU - Lin, Victor K.
N1 - Funding Information:
This study was supported by grants from the National Institutes of Health and the Muscular Dystrophy Association. The in situ hybridization experiments were performed in the laboratory of Dr. Margaret Buckingham at the Pasteur Institute, with support to M. 8. from the MDA, CNRS, ARC, and INSERM. We would like to thank Andrew Lassar and Charles Emerson for numerous helpful discussions and the sharing of probes and unpublished information. We would also like to thank Steven Sprang for help in comparing the predicted protein structures of myogenin and MyoD.
PY - 1989/2/24
Y1 - 1989/2/24
N2 - In this report, we describe the isolation, sequence, and initial characterization of the cDNA for the muscle-specific regulatory factor skeletal myogenin. Transfection of myogenin into the mesenchymal cell line C3H10T1/2 produces cells expressing muscle-specific markers. Myogenin is absent in undifferentiated cells, peaks, and then declines following a stimulus to differentiate, and is overexpressed in myoblasts selected with 5-bromodeoxyuridine for the overproduction of factors that regulate the decision to differentiate. High levels of myogenin transcripts are present in the myotomal region of somites at 8.5 days of gestation in the mouse. Although myogenin and MyoD are different genes, they share the myc homology domain. Myogenin and MyoD thus form part of a gene family regulating myogenesis, and together with myd may constitute a set of factors that interact to regulate the determination and differentiation of muscle cells.
AB - In this report, we describe the isolation, sequence, and initial characterization of the cDNA for the muscle-specific regulatory factor skeletal myogenin. Transfection of myogenin into the mesenchymal cell line C3H10T1/2 produces cells expressing muscle-specific markers. Myogenin is absent in undifferentiated cells, peaks, and then declines following a stimulus to differentiate, and is overexpressed in myoblasts selected with 5-bromodeoxyuridine for the overproduction of factors that regulate the decision to differentiate. High levels of myogenin transcripts are present in the myotomal region of somites at 8.5 days of gestation in the mouse. Although myogenin and MyoD are different genes, they share the myc homology domain. Myogenin and MyoD thus form part of a gene family regulating myogenesis, and together with myd may constitute a set of factors that interact to regulate the determination and differentiation of muscle cells.
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U2 - 10.1016/0092-8674(89)90583-7
DO - 10.1016/0092-8674(89)90583-7
M3 - Article
C2 - 2537150
AN - SCOPUS:0024576880
SN - 0092-8674
VL - 56
SP - 607
EP - 617
JO - Cell
JF - Cell
IS - 4
ER -