Myosin phosphorylation decreases the ATPase activity of cardiac myofibrils

Kathleen Franks, Roger Cooke, James T. Stull

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Our previous work showed that myosin phosphorylation decreased the ATPase activity of skeletal muscle myofibrils that were lightly fixed with glutaraldehyde. The fixation process prevented sarcomere shortening and destruction of the ordered filament array upon the addition of ATP. We have now extended these results to myofibrils prepared from hearts of rabbits, dogs and rats. Myofibrils were phosphorylated by incubation with myosin light chain kinase, calmodulin and either ATP-γs or ATP, for 15 minutes at 25°C. The extent of myosin light chain phosphorylation was 50% to 80%. The ATPase activity of unphosphorylated myofibrils was not altered by reaction with 0.01% glutaraldehyde for 5 minutes at 0°C, and the ATPase activity of unfixed myofibrils was not changed by phosphorylation. However, phosphorylation decreased the ATPase activity of fixed myofibrils by 50%. The effect on myocardial myofibrillar ATPase activity of phosphorylation was similar in the three animal species. These results suggest that in both skeletal and cardiac muscle, myosin phosphorylation decreases the rate of cross-bridge cycling resulting in decreased energy expenditure. It also appears that the effect of myosin light chain phosphorylation on ATPase activity requires an ordered myofilament structure.

Original languageEnglish (US)
Pages (from-to)597-604
Number of pages8
JournalJournal of Molecular and Cellular Cardiology
Volume16
Issue number7
DOIs
StatePublished - 1984

Fingerprint

Myofibrils
Myosins
Adenosine Triphosphatases
Phosphorylation
Myosin Light Chains
Adenosine Triphosphate
Glutaral
Skeletal Muscle Myosins
Cardiac Myosins
Myosin-Light-Chain Kinase
Sarcomeres
Calmodulin
Energy Metabolism
Skeletal Muscle
Dogs
Rabbits

Keywords

  • ATPase activity
  • Calcium ions
  • Calmodulin
  • Cardiac myofibrils
  • Myosin light chain kinase
  • Myosin phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Cardiology and Cardiovascular Medicine

Cite this

Myosin phosphorylation decreases the ATPase activity of cardiac myofibrils. / Franks, Kathleen; Cooke, Roger; Stull, James T.

In: Journal of Molecular and Cellular Cardiology, Vol. 16, No. 7, 1984, p. 597-604.

Research output: Contribution to journalArticle

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