Myristoylated alpha subunits of guanine nucleotide-binding regulatory proteins.

J. E. Buss, S. M. Mumby, P. J. Casey, A. G. Gilman, B. M. Sefton

Research output: Contribution to journalArticle

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Abstract

Antisera directed against specific subunits of guanine nucleotide-binding regulatory proteins (G proteins) were used to immunoprecipitate these polypeptides from metabolically labeled cells. This technique detects, in extracts of a human astrocytoma cell line, the alpha subunits of Gs (stimulatory) (alpha 45 and alpha 52), a 41-kDa subunit of Gi (inhibitory) (alpha 41), a 40-kDa protein (alpha 40), and the 36-kDa beta subunit. No protein that comigrated with the alpha subunit of Go (unknown function) (alpha 39) was detected. In cells grown in the presence of [3H]myristic acid, alpha 41 and alpha 40 contained 3H label, while the beta subunit did not. Chemical analysis of lipids attached covalently to purified alpha 41 and alpha 39 from bovine brain also revealed myristic acid. Similar analysis of brain G protein beta and gamma subunits and of Gt (transducin) subunits (alpha, beta, and gamma) failed to reveal fatty acids. The fatty acid associated with alpha 41, alpha 40, and alpha 39 was stable to treatment with base, suggesting that the lipid is linked to the polypeptide via an amide bond. These GTP binding proteins are thus identified as members of a select group of proteins that contains myristic acid covalently attached to the peptide backbone. Myristate may play an important role in stabilizing interactions of G proteins with phospholipid or with membrane-bound proteins.

Original languageEnglish (US)
Pages (from-to)7493-7497
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number21
StatePublished - Nov 1987

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GTP-Binding Proteins
Myristic Acid
Carrier Proteins
Gi-Go GTP-Binding Protein alpha Subunits
Proteins
Peptides
Fatty Acids
Gs GTP-Binding Protein alpha Subunits
Transducin
Lipids
Astrocytoma
Brain
Amides
Immune Sera
Phospholipids
Membrane Proteins
Cell Line

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Myristoylated alpha subunits of guanine nucleotide-binding regulatory proteins. / Buss, J. E.; Mumby, S. M.; Casey, P. J.; Gilman, A. G.; Sefton, B. M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, No. 21, 11.1987, p. 7493-7497.

Research output: Contribution to journalArticle

Buss, J. E. ; Mumby, S. M. ; Casey, P. J. ; Gilman, A. G. ; Sefton, B. M. / Myristoylated alpha subunits of guanine nucleotide-binding regulatory proteins. In: Proceedings of the National Academy of Sciences of the United States of America. 1987 ; Vol. 84, No. 21. pp. 7493-7497.
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