N-acetylaspartate complexes with calcium and lanthanide ions

Y. Rubin, G. P. Connelly, R. E. Lenkinski

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

N-acetylaspartate (NAA) is the one of the most prominent resonances observed in the solvent-suppressed NMR spectrum of the human brain. Although it is present in the brain at about 10 mM, its precise metabolic function is still unclear, We have examined the NAA as a potential chelator for divalent metal ions such as Ca2+. We have employed the perturbations induced by Ln3+ ions in the 1H and 13C NMR spectrum of NAA to monitor formation of NAA complexes. 1 NMR measurements showed that the dissociation constants for the formation of Eu3+-NAA, Yb3+-NAA, and Ca2+-NAA complexes were 0.07, 0.13, and 0.86 mM, respectively. Scatchard analysis of the results indicates the formation of a 1:1 metal-ligand complex. We also inferred the structure of the NAA-metal ion complex from a analysis of paramagnetic perturbations induced in the 1H NMR and 13C NMR spectra of NAA. The structural analysis of the NAA-metal ion complex indicates that the two carboxylic groups participate in chelating the metal ion, forming the binding site for the metal ion.

Original languageEnglish (US)
Pages (from-to)31-43
Number of pages13
JournalJournal of Inorganic Biochemistry
Volume60
Issue number1
DOIs
StatePublished - Oct 1995

ASJC Scopus subject areas

  • Biochemistry
  • Inorganic Chemistry

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