Nanofabrication of protein-patterned substrates for future cell adhesion experiments

P. A. Künzi, J. Lussi, L. Aeschimann, G. Danuser, M. Textor, N. F. De Rooij, U. Staufer

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

A method for fabricating sub-micrometer size adhesion sites for future experiments in cell biology is presented. Glass substrates were coated with a thin layer of InSnO and SiO2. The SiO2 was structured by means of electron beam lithography and reactive ion etching, exposing sub-micrometer patches of the underlying InSnO. Dodecylphosphate, to which proteins can bind, was selectively adsorbed on these InSnO structures, whereas poly-l-lysine-g-poly(ethylene glycol) was used to passivate the surrounding SiO2 against protein adsorption. The effectiveness of the process was investigated by fluorescent microscopy and scanning near-field optical microscopy on substrates which have been exposed to fluorescently labeled streptavidin.

Original languageEnglish (US)
Pages (from-to)582-586
Number of pages5
JournalMicroelectronic Engineering
Volume78-79
Issue number1-4
DOIs
StatePublished - Mar 2005

Fingerprint

nanofabrication
Cell adhesion
Nanotechnology
micrometers
adhesion
Cytology
microscopy
Near field scanning optical microscopy
proteins
Proteins
Streptavidin
Electron beam lithography
lysine
Reactive ion etching
Substrates
cells
Polyethylene glycols
Lysine
glycols
near fields

Keywords

  • Electron beam lithography
  • Indium-tin-oxide
  • Nanofabrication
  • Protein adsorption
  • Protein patterning
  • Self-assembling

ASJC Scopus subject areas

  • Hardware and Architecture
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Surfaces, Coatings and Films
  • Atomic and Molecular Physics, and Optics

Cite this

Künzi, P. A., Lussi, J., Aeschimann, L., Danuser, G., Textor, M., De Rooij, N. F., & Staufer, U. (2005). Nanofabrication of protein-patterned substrates for future cell adhesion experiments. Microelectronic Engineering, 78-79(1-4), 582-586. https://doi.org/10.1016/j.mee.2004.12.073

Nanofabrication of protein-patterned substrates for future cell adhesion experiments. / Künzi, P. A.; Lussi, J.; Aeschimann, L.; Danuser, G.; Textor, M.; De Rooij, N. F.; Staufer, U.

In: Microelectronic Engineering, Vol. 78-79, No. 1-4, 03.2005, p. 582-586.

Research output: Contribution to journalArticle

Künzi, PA, Lussi, J, Aeschimann, L, Danuser, G, Textor, M, De Rooij, NF & Staufer, U 2005, 'Nanofabrication of protein-patterned substrates for future cell adhesion experiments', Microelectronic Engineering, vol. 78-79, no. 1-4, pp. 582-586. https://doi.org/10.1016/j.mee.2004.12.073
Künzi, P. A. ; Lussi, J. ; Aeschimann, L. ; Danuser, G. ; Textor, M. ; De Rooij, N. F. ; Staufer, U. / Nanofabrication of protein-patterned substrates for future cell adhesion experiments. In: Microelectronic Engineering. 2005 ; Vol. 78-79, No. 1-4. pp. 582-586.
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