Nanoscale bio-platforms for living cell interrogation: Current status and future perspectives

Lingqian Chang, Jiaming Hu, Feng Chen, Zhou Chen, Junfeng Shi, Zhaogang Yang, Yiwen Li, Ly James Lee

Research output: Contribution to journalReview article

28 Scopus citations


The living cell is a complex entity that dynamically responds to both intracellular and extracellular environments. Extensive efforts have been devoted to the understanding intracellular functions orchestrated with mRNAs and proteins in investigation of the fate of a single-cell, including proliferation, apoptosis, motility, differentiation and mutations. The rapid development of modern cellular analysis techniques (e.g. PCR, western blotting, immunochemistry, etc.) offers new opportunities in quantitative analysis of RNA/protein expression up to a single cell level. The recent entries of nanoscale platforms that include kinds of methodologies with high spatial and temporal resolution have been widely employed to probe the living cells. In this tutorial review paper, we give insight into background introduction and technical innovation of currently reported nanoscale platforms for living cell interrogation. These highlighted technologies are documented in details within four categories, including nano-biosensors for label-free detection of living cells, nanodevices for living cell probing by intracellular marker delivery, high-throughput platforms towards clinical current, and the progress of microscopic imaging platforms for cell/tissue tracking in vitro and in vivo. Perspectives for system improvement were also discussed to solve the limitations remains in current techniques, for the purpose of clinical use in future.

Original languageEnglish (US)
Pages (from-to)3181-3206
Number of pages26
Issue number6
StatePublished - Feb 14 2016
Externally publishedYes


ASJC Scopus subject areas

  • Materials Science(all)

Cite this

Chang, L., Hu, J., Chen, F., Chen, Z., Shi, J., Yang, Z., Li, Y., & Lee, L. J. (2016). Nanoscale bio-platforms for living cell interrogation: Current status and future perspectives. Nanoscale, 8(6), 3181-3206.