Nanotextured substrates with immobilized aptamers for cancer cell isolation and cytology

Yuan Wan, M. Arif Iftakher Mahmood, Na Li, Peter B. Allen, Young Tae Kim, Robert Bachoo, Andrew D. Ellington, Samir M. Iqbal

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Background: The detection of a small number of circulating tumor cells (CTCs) is important, especially in the early stages of cancer. Small numbers of CTCs are hard to detect, because very few approaches are sensitive enough to differentiate these from the pool of other cells. Improving the affinity of a selective, surface-functionalized molecule is important given the scarcity of CTCs in vivo. There are several proteins and aptamers that provide such high affinity; however, using surface nanotexturing increases this affinity even further. Methods: The authors report an approach to improve the affinity of tumor cell capture by using novel aptamers against cell membrane overexpressed epidermal growth factor receptors (EGFRs) on a nanotextured polydimethylsiloxane (PDMS) substrate. Surface-immobilized aptamers were used to specifically capture tumor cells from physiologic samples. Results: The nanotexturing of PDMS increased surface roughness at the nanoscale. This increased the effective surface area and resulted in a significantly higher degree of surface functionalization. The phenomenon resulted in increased density of immobilized EGFR-specific RNA aptamer molecules and provided significantly higher efficiency to capture cancer cells from a mixture. The data indicated that CTCs could be captured and enriched, leading to higher yield yet higher background. Conclusions: A comparison between glass slides, plain PDMS, and nanotextured PDMS functionalized with aptamers demonstrated that a 2-fold approach of using aptamers on nanotextured PDMS can be important for cancer cytology devices, and especially for the idea of a "lab-on-chip," toward higher yield in capture efficiency.

Original languageEnglish (US)
Pages (from-to)1145-1154
Number of pages10
JournalCancer
Volume118
Issue number4
DOIs
StatePublished - Feb 15 2012

Fingerprint

Cell Separation
Circulating Neoplastic Cells
Cell Biology
Neoplasms
Nucleotide Aptamers
Glass
Cell Membrane
baysilon
Equipment and Supplies
Proteins
ErbB Receptors

Keywords

  • basement membrane
  • circulating tumor cells
  • human glioblastoma
  • lab-on-chip
  • microscopy
  • nanotextured materials
  • polydimethylsiloxane
  • RNA aptamers

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Wan, Y., Mahmood, M. A. I., Li, N., Allen, P. B., Kim, Y. T., Bachoo, R., ... Iqbal, S. M. (2012). Nanotextured substrates with immobilized aptamers for cancer cell isolation and cytology. Cancer, 118(4), 1145-1154. https://doi.org/10.1002/cncr.26349

Nanotextured substrates with immobilized aptamers for cancer cell isolation and cytology. / Wan, Yuan; Mahmood, M. Arif Iftakher; Li, Na; Allen, Peter B.; Kim, Young Tae; Bachoo, Robert; Ellington, Andrew D.; Iqbal, Samir M.

In: Cancer, Vol. 118, No. 4, 15.02.2012, p. 1145-1154.

Research output: Contribution to journalArticle

Wan, Y, Mahmood, MAI, Li, N, Allen, PB, Kim, YT, Bachoo, R, Ellington, AD & Iqbal, SM 2012, 'Nanotextured substrates with immobilized aptamers for cancer cell isolation and cytology', Cancer, vol. 118, no. 4, pp. 1145-1154. https://doi.org/10.1002/cncr.26349
Wan, Yuan ; Mahmood, M. Arif Iftakher ; Li, Na ; Allen, Peter B. ; Kim, Young Tae ; Bachoo, Robert ; Ellington, Andrew D. ; Iqbal, Samir M. / Nanotextured substrates with immobilized aptamers for cancer cell isolation and cytology. In: Cancer. 2012 ; Vol. 118, No. 4. pp. 1145-1154.
@article{268db1968e81452f97602c0706de5d2b,
title = "Nanotextured substrates with immobilized aptamers for cancer cell isolation and cytology",
abstract = "Background: The detection of a small number of circulating tumor cells (CTCs) is important, especially in the early stages of cancer. Small numbers of CTCs are hard to detect, because very few approaches are sensitive enough to differentiate these from the pool of other cells. Improving the affinity of a selective, surface-functionalized molecule is important given the scarcity of CTCs in vivo. There are several proteins and aptamers that provide such high affinity; however, using surface nanotexturing increases this affinity even further. Methods: The authors report an approach to improve the affinity of tumor cell capture by using novel aptamers against cell membrane overexpressed epidermal growth factor receptors (EGFRs) on a nanotextured polydimethylsiloxane (PDMS) substrate. Surface-immobilized aptamers were used to specifically capture tumor cells from physiologic samples. Results: The nanotexturing of PDMS increased surface roughness at the nanoscale. This increased the effective surface area and resulted in a significantly higher degree of surface functionalization. The phenomenon resulted in increased density of immobilized EGFR-specific RNA aptamer molecules and provided significantly higher efficiency to capture cancer cells from a mixture. The data indicated that CTCs could be captured and enriched, leading to higher yield yet higher background. Conclusions: A comparison between glass slides, plain PDMS, and nanotextured PDMS functionalized with aptamers demonstrated that a 2-fold approach of using aptamers on nanotextured PDMS can be important for cancer cytology devices, and especially for the idea of a {"}lab-on-chip,{"} toward higher yield in capture efficiency.",
keywords = "basement membrane, circulating tumor cells, human glioblastoma, lab-on-chip, microscopy, nanotextured materials, polydimethylsiloxane, RNA aptamers",
author = "Yuan Wan and Mahmood, {M. Arif Iftakher} and Na Li and Allen, {Peter B.} and Kim, {Young Tae} and Robert Bachoo and Ellington, {Andrew D.} and Iqbal, {Samir M.}",
year = "2012",
month = "2",
day = "15",
doi = "10.1002/cncr.26349",
language = "English (US)",
volume = "118",
pages = "1145--1154",
journal = "Cancer",
issn = "0008-543X",
publisher = "John Wiley and Sons Inc.",
number = "4",

}

TY - JOUR

T1 - Nanotextured substrates with immobilized aptamers for cancer cell isolation and cytology

AU - Wan, Yuan

AU - Mahmood, M. Arif Iftakher

AU - Li, Na

AU - Allen, Peter B.

AU - Kim, Young Tae

AU - Bachoo, Robert

AU - Ellington, Andrew D.

AU - Iqbal, Samir M.

PY - 2012/2/15

Y1 - 2012/2/15

N2 - Background: The detection of a small number of circulating tumor cells (CTCs) is important, especially in the early stages of cancer. Small numbers of CTCs are hard to detect, because very few approaches are sensitive enough to differentiate these from the pool of other cells. Improving the affinity of a selective, surface-functionalized molecule is important given the scarcity of CTCs in vivo. There are several proteins and aptamers that provide such high affinity; however, using surface nanotexturing increases this affinity even further. Methods: The authors report an approach to improve the affinity of tumor cell capture by using novel aptamers against cell membrane overexpressed epidermal growth factor receptors (EGFRs) on a nanotextured polydimethylsiloxane (PDMS) substrate. Surface-immobilized aptamers were used to specifically capture tumor cells from physiologic samples. Results: The nanotexturing of PDMS increased surface roughness at the nanoscale. This increased the effective surface area and resulted in a significantly higher degree of surface functionalization. The phenomenon resulted in increased density of immobilized EGFR-specific RNA aptamer molecules and provided significantly higher efficiency to capture cancer cells from a mixture. The data indicated that CTCs could be captured and enriched, leading to higher yield yet higher background. Conclusions: A comparison between glass slides, plain PDMS, and nanotextured PDMS functionalized with aptamers demonstrated that a 2-fold approach of using aptamers on nanotextured PDMS can be important for cancer cytology devices, and especially for the idea of a "lab-on-chip," toward higher yield in capture efficiency.

AB - Background: The detection of a small number of circulating tumor cells (CTCs) is important, especially in the early stages of cancer. Small numbers of CTCs are hard to detect, because very few approaches are sensitive enough to differentiate these from the pool of other cells. Improving the affinity of a selective, surface-functionalized molecule is important given the scarcity of CTCs in vivo. There are several proteins and aptamers that provide such high affinity; however, using surface nanotexturing increases this affinity even further. Methods: The authors report an approach to improve the affinity of tumor cell capture by using novel aptamers against cell membrane overexpressed epidermal growth factor receptors (EGFRs) on a nanotextured polydimethylsiloxane (PDMS) substrate. Surface-immobilized aptamers were used to specifically capture tumor cells from physiologic samples. Results: The nanotexturing of PDMS increased surface roughness at the nanoscale. This increased the effective surface area and resulted in a significantly higher degree of surface functionalization. The phenomenon resulted in increased density of immobilized EGFR-specific RNA aptamer molecules and provided significantly higher efficiency to capture cancer cells from a mixture. The data indicated that CTCs could be captured and enriched, leading to higher yield yet higher background. Conclusions: A comparison between glass slides, plain PDMS, and nanotextured PDMS functionalized with aptamers demonstrated that a 2-fold approach of using aptamers on nanotextured PDMS can be important for cancer cytology devices, and especially for the idea of a "lab-on-chip," toward higher yield in capture efficiency.

KW - basement membrane

KW - circulating tumor cells

KW - human glioblastoma

KW - lab-on-chip

KW - microscopy

KW - nanotextured materials

KW - polydimethylsiloxane

KW - RNA aptamers

UR - http://www.scopus.com/inward/record.url?scp=84856808809&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856808809&partnerID=8YFLogxK

U2 - 10.1002/cncr.26349

DO - 10.1002/cncr.26349

M3 - Article

VL - 118

SP - 1145

EP - 1154

JO - Cancer

JF - Cancer

SN - 0008-543X

IS - 4

ER -