Nanotubes mediate niche-stem-cell signalling in the Drosophila testis

Mayu Inaba, Michael Buszczak, Yukiko M. Yamashita

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Stem cell niches provide resident stem cells with signals that specify their identity. Niche signals act over a short range such that only stem cells but not their differentiating progeny receive the self-renewing signals. However, the cellular mechanisms that limit niche signalling to stem cells remain poorly understood. Here we show that the Drosophila male germline stem cells form previously unrecognized structures, microtubule-based nanotubes, which extend into the hub, a major niche component. Microtubule-based nanotubes are observed specifically within germline stem cell populations, and require intraflagellar transport proteins for their formation. The bone morphogenetic protein (BMP) receptor Tkv localizes to microtubule-based nanotubes. Perturbation of microtubule-based nanotubes compromises activation of Dpp signalling within germline stem cells, leading to germline stem cell loss. Moreover, Dpp ligand and Tkv receptor interaction is necessary and sufficient for microtubule-based nanotube formation. We propose that microtubule-based nanotubes provide a novel mechanism for selective receptor-ligand interaction, contributing to the short-range nature of niche-stem-cell signalling.

Original languageEnglish (US)
Pages (from-to)329-332
Number of pages4
JournalNature
Volume523
Issue number7560
DOIs
StatePublished - Jul 16 2015

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Stem Cell Niche
Nanotubes
Drosophila
Testis
Microtubules
Stem Cells
Bone Morphogenetic Protein Receptors
Ligands
Carrier Proteins

ASJC Scopus subject areas

  • General

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Nanotubes mediate niche-stem-cell signalling in the Drosophila testis. / Inaba, Mayu; Buszczak, Michael; Yamashita, Yukiko M.

In: Nature, Vol. 523, No. 7560, 16.07.2015, p. 329-332.

Research output: Contribution to journalArticle

Inaba, Mayu ; Buszczak, Michael ; Yamashita, Yukiko M. / Nanotubes mediate niche-stem-cell signalling in the Drosophila testis. In: Nature. 2015 ; Vol. 523, No. 7560. pp. 329-332.
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