Nardosinone protects H9c2 cardiac cells from angiotensin II-induced hypertrophy

Meng Du, Kun Huang, Lu Gao, Liu Yang, Wen Shuo Wang, Bo Wang, Kai Huang, Dan Huang

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Pathological cardiac hypertrophy induced by angiotensin II (AngII) can subsequently give rise to heart failure, a leading cause of mortality. Nardosinone is a pharmacologically active compound extracted from the roots of Nardostachys chinensis, a well-known traditional Chinese medicine. In order to investigate the effects of nardosinone on AngII-induced cardiac cell hypertrophy and the related mechanisms, the myoblast cell line H9c2, derived from embryonic rat heart, was treated with nardosinone (25, 50, 100, and 200 μmol/L) or AngII (1 μmol/L). Then cell surface area and mRNA expression of classical markers of hypertrophy were detected. The related protein levels in PI3K/Akt/mTOR and MEK/ERK signaling pathways were examined by Western blotting. It was found that pretreatment with nardosinone could significantly inhibit the enlargement of cell surface area induced by AngII. The mRNA expression of ANP, BNP and β-MHC was obviously elevated in AngII-treated H9c2 cells, which could be effectively blocked by nardosinone at the concentration of 100 μmol/L. Further study revealed that the protective effects of nardosinone might be mediated by repressing the phosphorylation of related proteins in PI3K/Akt and MEK/ERK signaling pathways. It was suggested that the inhibitory effect of nardosinone on Ang II-induced hypertrophy in H9c2 cells might be mediated by targeting PI3K/Akt and MEK/ERK signaling pathways.

Original languageEnglish (US)
Pages (from-to)822-826
Number of pages5
JournalJournal of Huazhong University of Science and Technology - Medical Science
Volume33
Issue number6
DOIs
StatePublished - Dec 1 2013
Externally publishedYes

Keywords

  • cardiac hypertrophy
  • H9c2 cells
  • MEK/ERK
  • nardosinone
  • PI3K/Akt

ASJC Scopus subject areas

  • Biochemistry
  • Biomaterials
  • Biomedical Engineering
  • Genetics

Fingerprint Dive into the research topics of 'Nardosinone protects H9c2 cardiac cells from angiotensin II-induced hypertrophy'. Together they form a unique fingerprint.

  • Cite this