Abstract
The Na+/Ca2+ exchanger (NCX1) plays a key role in maintaining Ca2+ homeostasis in cardiomyocytes. Disruption of Ncx1 gene in mice results in embryonic lethality between embryonic day 9 and 10, with the mice lacking spontaneous heartbeats. We examined the mechanism of lack of heartbeats in Ncx1-deficient mice. Ultrastructual analysis demonstrated that Ncx1-deficient mice showed severe disorganization of myofibrils, a lack of Z-lines and swelling of mitochondria in cardiomyocytes. However, the expressions of cardiac-specific genes including transcription factor genes and contractile protein genes were not changed in Ncx1-deficient mice. Abnormal Ca2+ handling itself or the lack of heartbeats due to the inactivation of Ncx1 gene may cause the disorganization of myofibrillogenesis. Although NCX1 protein levels were decreased in heterozygous mice, there were no changes in NCX2 and NCX3 protein levels between wild type and heterozygous mice.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 9-15 |
| Number of pages | 7 |
| Journal | Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology |
| Volume | 135 |
| Issue number | 1 |
| DOIs | |
| State | Published - May 1 2003 |
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Keywords
- Embryo
- Heart
- Heartbeat
- Mitochondria
- Mouse
- Myofibril
- Na/Ca exchanger
- NCX
ASJC Scopus subject areas
- Biochemistry
- Physiology
Cite this
Na+/Ca2+ exchanger-deficient mice have disorganized myofibrils and swollen mitochondria in cardiomyocytes. / Wakimoto, Koji; Fujimura, Hisako; Iwamoto, Takahiro; Oka, Toru; Kobayashi, Kinji; Kita, Satomi; Kudoh, Sumiyo; Kuro-o, Makoto; Nabeshima, Yo Ichi; Shigekawa, Munekazu; Imai, Yuji; Komuro, Issei.
In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 135, No. 1, 01.05.2003, p. 9-15.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Na+/Ca2+ exchanger-deficient mice have disorganized myofibrils and swollen mitochondria in cardiomyocytes
AU - Wakimoto, Koji
AU - Fujimura, Hisako
AU - Iwamoto, Takahiro
AU - Oka, Toru
AU - Kobayashi, Kinji
AU - Kita, Satomi
AU - Kudoh, Sumiyo
AU - Kuro-o, Makoto
AU - Nabeshima, Yo Ichi
AU - Shigekawa, Munekazu
AU - Imai, Yuji
AU - Komuro, Issei
PY - 2003/5/1
Y1 - 2003/5/1
N2 - The Na+/Ca2+ exchanger (NCX1) plays a key role in maintaining Ca2+ homeostasis in cardiomyocytes. Disruption of Ncx1 gene in mice results in embryonic lethality between embryonic day 9 and 10, with the mice lacking spontaneous heartbeats. We examined the mechanism of lack of heartbeats in Ncx1-deficient mice. Ultrastructual analysis demonstrated that Ncx1-deficient mice showed severe disorganization of myofibrils, a lack of Z-lines and swelling of mitochondria in cardiomyocytes. However, the expressions of cardiac-specific genes including transcription factor genes and contractile protein genes were not changed in Ncx1-deficient mice. Abnormal Ca2+ handling itself or the lack of heartbeats due to the inactivation of Ncx1 gene may cause the disorganization of myofibrillogenesis. Although NCX1 protein levels were decreased in heterozygous mice, there were no changes in NCX2 and NCX3 protein levels between wild type and heterozygous mice.
AB - The Na+/Ca2+ exchanger (NCX1) plays a key role in maintaining Ca2+ homeostasis in cardiomyocytes. Disruption of Ncx1 gene in mice results in embryonic lethality between embryonic day 9 and 10, with the mice lacking spontaneous heartbeats. We examined the mechanism of lack of heartbeats in Ncx1-deficient mice. Ultrastructual analysis demonstrated that Ncx1-deficient mice showed severe disorganization of myofibrils, a lack of Z-lines and swelling of mitochondria in cardiomyocytes. However, the expressions of cardiac-specific genes including transcription factor genes and contractile protein genes were not changed in Ncx1-deficient mice. Abnormal Ca2+ handling itself or the lack of heartbeats due to the inactivation of Ncx1 gene may cause the disorganization of myofibrillogenesis. Although NCX1 protein levels were decreased in heterozygous mice, there were no changes in NCX2 and NCX3 protein levels between wild type and heterozygous mice.
KW - Embryo
KW - Heart
KW - Heartbeat
KW - Mitochondria
KW - Mouse
KW - Myofibril
KW - Na/Ca exchanger
KW - NCX
UR - http://www.scopus.com/inward/record.url?scp=0037683719&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0037683719&partnerID=8YFLogxK
U2 - 10.1016/S1096-4959(03)00057-5
DO - 10.1016/S1096-4959(03)00057-5
M3 - Article
C2 - 12781968
AN - SCOPUS:0037683719
VL - 135
SP - 9
EP - 15
JO - Comparative biochemistry and physiology. B, Comparative biochemistry
JF - Comparative biochemistry and physiology. B, Comparative biochemistry
SN - 1096-4959
IS - 1
ER -