Na+/H+ antiporter (NHE-1 isoform) in cultured vascular smooth muscle from the spontaneously hypertensive rat

M. S. LaPointe, M. Ye, O. W. Moe, R. J. Alpern, D. C. Batlle

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

An increase in Na+/H+ antiporter activity may be involved in hyperproliferation of vascular smooth muscle cells (VSMC) and possibly in the vascular hyperplasia characteristic of hypertension. The present study was designed to examine cell proliferation, Na+/H+ exchange activity, and mRNA levels of the NHE-1 isoform of the Na+/H+ antiporter in cultured aortic VSMC derived from the spontaneously hypertensive rat (SHR) and from normotensive controls, the Wistar/Kyoto rat (WKY). VSMC derived from the SHR grown in early (2 to 6), but not in later (7 to 10) sub-passages, exhibited an increase in [3H]-thymidine incorporation and shorter doubling times as compared to those derived from WKY rats. Na+/H+ exchange activity assayed in the nominal absence of HCO3 -/CO2, as the rate of intracellular pH (pH(i)) recovery after cell acidification was significantly higher in cells from SHR than in those from WKY rats when cells were studied in early sub-passages, but not in cells studied in later sub-passages. In cells grown in early sub-passage, Na+/H+ exchange activity assessed as the initial rate of Na+(i) accumulation following acute cell acidification was also significantly higher in SHR than WKY cells both in the nominal absence (10.22 ± 1.15 and 6.98 ± 1.17 mmol Na+(i)/90 seconds, P < 0.05, respectively) and in the presence of HCO3 -/CO2 (9.94 ± 1.02 and 5.59 ± 0.86 mmol Na+/90 seconds, P < 0.01, respectively). There were no detectable differences in the level of steady-state Na+/H+ antiporter (NHE-1) mRNA between VSMC from SHR and WKY rats. Our findings indicate that Na+/H+ exchange activity is increased in cultured aortic VSMC derived from SHR as compared to those derived from WKY rats. The higher functional activity of the Na+/H+ antiporter in VSMC from the SHR is due to a post-transcriptional event(s) and may be related to enhanced growth in culture.

Original languageEnglish (US)
Pages (from-to)78-87
Number of pages10
JournalKidney International
Volume47
Issue number1
StatePublished - 1995

Fingerprint

Sodium-Hydrogen Antiporter
Inbred SHR Rats
Inbred WKY Rats
Vascular Smooth Muscle
Protein Isoforms
Smooth Muscle Myocytes
Messenger RNA
Thymidine
Hyperplasia
Blood Vessels
Cell Proliferation
Hypertension
Growth

ASJC Scopus subject areas

  • Nephrology

Cite this

Na+/H+ antiporter (NHE-1 isoform) in cultured vascular smooth muscle from the spontaneously hypertensive rat. / LaPointe, M. S.; Ye, M.; Moe, O. W.; Alpern, R. J.; Batlle, D. C.

In: Kidney International, Vol. 47, No. 1, 1995, p. 78-87.

Research output: Contribution to journalArticle

LaPointe, M. S. ; Ye, M. ; Moe, O. W. ; Alpern, R. J. ; Batlle, D. C. / Na+/H+ antiporter (NHE-1 isoform) in cultured vascular smooth muscle from the spontaneously hypertensive rat. In: Kidney International. 1995 ; Vol. 47, No. 1. pp. 78-87.
@article{f095a75073804614882192e759ea4ca9,
title = "Na+/H+ antiporter (NHE-1 isoform) in cultured vascular smooth muscle from the spontaneously hypertensive rat",
abstract = "An increase in Na+/H+ antiporter activity may be involved in hyperproliferation of vascular smooth muscle cells (VSMC) and possibly in the vascular hyperplasia characteristic of hypertension. The present study was designed to examine cell proliferation, Na+/H+ exchange activity, and mRNA levels of the NHE-1 isoform of the Na+/H+ antiporter in cultured aortic VSMC derived from the spontaneously hypertensive rat (SHR) and from normotensive controls, the Wistar/Kyoto rat (WKY). VSMC derived from the SHR grown in early (2 to 6), but not in later (7 to 10) sub-passages, exhibited an increase in [3H]-thymidine incorporation and shorter doubling times as compared to those derived from WKY rats. Na+/H+ exchange activity assayed in the nominal absence of HCO3 -/CO2, as the rate of intracellular pH (pH(i)) recovery after cell acidification was significantly higher in cells from SHR than in those from WKY rats when cells were studied in early sub-passages, but not in cells studied in later sub-passages. In cells grown in early sub-passage, Na+/H+ exchange activity assessed as the initial rate of Na+(i) accumulation following acute cell acidification was also significantly higher in SHR than WKY cells both in the nominal absence (10.22 ± 1.15 and 6.98 ± 1.17 mmol Na+(i)/90 seconds, P < 0.05, respectively) and in the presence of HCO3 -/CO2 (9.94 ± 1.02 and 5.59 ± 0.86 mmol Na+/90 seconds, P < 0.01, respectively). There were no detectable differences in the level of steady-state Na+/H+ antiporter (NHE-1) mRNA between VSMC from SHR and WKY rats. Our findings indicate that Na+/H+ exchange activity is increased in cultured aortic VSMC derived from SHR as compared to those derived from WKY rats. The higher functional activity of the Na+/H+ antiporter in VSMC from the SHR is due to a post-transcriptional event(s) and may be related to enhanced growth in culture.",
author = "LaPointe, {M. S.} and M. Ye and Moe, {O. W.} and Alpern, {R. J.} and Batlle, {D. C.}",
year = "1995",
language = "English (US)",
volume = "47",
pages = "78--87",
journal = "Kidney International",
issn = "0085-2538",
publisher = "Nature Publishing Group",
number = "1",

}

TY - JOUR

T1 - Na+/H+ antiporter (NHE-1 isoform) in cultured vascular smooth muscle from the spontaneously hypertensive rat

AU - LaPointe, M. S.

AU - Ye, M.

AU - Moe, O. W.

AU - Alpern, R. J.

AU - Batlle, D. C.

PY - 1995

Y1 - 1995

N2 - An increase in Na+/H+ antiporter activity may be involved in hyperproliferation of vascular smooth muscle cells (VSMC) and possibly in the vascular hyperplasia characteristic of hypertension. The present study was designed to examine cell proliferation, Na+/H+ exchange activity, and mRNA levels of the NHE-1 isoform of the Na+/H+ antiporter in cultured aortic VSMC derived from the spontaneously hypertensive rat (SHR) and from normotensive controls, the Wistar/Kyoto rat (WKY). VSMC derived from the SHR grown in early (2 to 6), but not in later (7 to 10) sub-passages, exhibited an increase in [3H]-thymidine incorporation and shorter doubling times as compared to those derived from WKY rats. Na+/H+ exchange activity assayed in the nominal absence of HCO3 -/CO2, as the rate of intracellular pH (pH(i)) recovery after cell acidification was significantly higher in cells from SHR than in those from WKY rats when cells were studied in early sub-passages, but not in cells studied in later sub-passages. In cells grown in early sub-passage, Na+/H+ exchange activity assessed as the initial rate of Na+(i) accumulation following acute cell acidification was also significantly higher in SHR than WKY cells both in the nominal absence (10.22 ± 1.15 and 6.98 ± 1.17 mmol Na+(i)/90 seconds, P < 0.05, respectively) and in the presence of HCO3 -/CO2 (9.94 ± 1.02 and 5.59 ± 0.86 mmol Na+/90 seconds, P < 0.01, respectively). There were no detectable differences in the level of steady-state Na+/H+ antiporter (NHE-1) mRNA between VSMC from SHR and WKY rats. Our findings indicate that Na+/H+ exchange activity is increased in cultured aortic VSMC derived from SHR as compared to those derived from WKY rats. The higher functional activity of the Na+/H+ antiporter in VSMC from the SHR is due to a post-transcriptional event(s) and may be related to enhanced growth in culture.

AB - An increase in Na+/H+ antiporter activity may be involved in hyperproliferation of vascular smooth muscle cells (VSMC) and possibly in the vascular hyperplasia characteristic of hypertension. The present study was designed to examine cell proliferation, Na+/H+ exchange activity, and mRNA levels of the NHE-1 isoform of the Na+/H+ antiporter in cultured aortic VSMC derived from the spontaneously hypertensive rat (SHR) and from normotensive controls, the Wistar/Kyoto rat (WKY). VSMC derived from the SHR grown in early (2 to 6), but not in later (7 to 10) sub-passages, exhibited an increase in [3H]-thymidine incorporation and shorter doubling times as compared to those derived from WKY rats. Na+/H+ exchange activity assayed in the nominal absence of HCO3 -/CO2, as the rate of intracellular pH (pH(i)) recovery after cell acidification was significantly higher in cells from SHR than in those from WKY rats when cells were studied in early sub-passages, but not in cells studied in later sub-passages. In cells grown in early sub-passage, Na+/H+ exchange activity assessed as the initial rate of Na+(i) accumulation following acute cell acidification was also significantly higher in SHR than WKY cells both in the nominal absence (10.22 ± 1.15 and 6.98 ± 1.17 mmol Na+(i)/90 seconds, P < 0.05, respectively) and in the presence of HCO3 -/CO2 (9.94 ± 1.02 and 5.59 ± 0.86 mmol Na+/90 seconds, P < 0.01, respectively). There were no detectable differences in the level of steady-state Na+/H+ antiporter (NHE-1) mRNA between VSMC from SHR and WKY rats. Our findings indicate that Na+/H+ exchange activity is increased in cultured aortic VSMC derived from SHR as compared to those derived from WKY rats. The higher functional activity of the Na+/H+ antiporter in VSMC from the SHR is due to a post-transcriptional event(s) and may be related to enhanced growth in culture.

UR - http://www.scopus.com/inward/record.url?scp=0028939182&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028939182&partnerID=8YFLogxK

M3 - Article

C2 - 7731174

AN - SCOPUS:0028939182

VL - 47

SP - 78

EP - 87

JO - Kidney International

JF - Kidney International

SN - 0085-2538

IS - 1

ER -