Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels

Yang Pu; Jianpeng Xue; Wubao Wang; Baogang Xu; Yueqing Gu; Rui Tang; Ellen Ackerstaff; Jason A. Koutcher; Samuel Achilefu; Robert R. Alfano

doi:10.1117/1.JBO.18.8.087002

Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels

Yang Pu, Jianpeng Xue, Wubao Wang, Baogang Xu, Yueqing Gu, Rui Tang, Ellen Ackerstaff, Jason A. Koutcher, Samuel Achilefu, Robert R. Alfano

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells.

Original language	English (US)
Article number	087002
Journal	Journal of biomedical optics
Volume	18
Issue number	8
DOIs	https://doi.org/10.1117/1.JBO.18.8.087002
State	Published - Aug 2013
Externally published	Yes

Keywords

NADH
metastasisrelevant key fluorophores
moderately metastatic and advanced metastatic cell lines
native fluorescence spectra
normal
optical biopsy
tryptophan

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Biomedical Engineering

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10.1117/1.JBO.18.8.087002

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@article{986049e581cf431eb8e5191ea1e59da2,

title = "Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels",

abstract = "The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells.",

keywords = "NADH, metastasisrelevant key fluorophores, moderately metastatic and advanced metastatic cell lines, native fluorescence spectra, normal, optical biopsy, tryptophan",

author = "Yang Pu and Jianpeng Xue and Wubao Wang and Baogang Xu and Yueqing Gu and Rui Tang and Ellen Ackerstaff and Koutcher, {Jason A.} and Samuel Achilefu and Alfano, {Robert R.}",

note = "Funding Information: This research is supported in part by U.S. Army Medical Research and Material Command grants numbered W81XWH-11-1-0335 and W81XWH-08-1-0717, and the National Institutes of Health (NIBIB R01 EB008111 and NCI R01 CA171651). Yang Pu thanks deeply Ms. Brenda Phelps at the administration of the Optical Radiology Lab at Washington University School of Medicine for her help and support during his working on this project at Washington University. Yang Pu also acknowledges Ms. Natalia Kruchevsky at Memorial Sloan Kettering Cancer Center (MSKCC) for her help in cell culture techniques.",

year = "2013",

month = aug,

doi = "10.1117/1.JBO.18.8.087002",

language = "English (US)",

volume = "18",

journal = "Journal of biomedical optics",

issn = "1083-3668",

publisher = "SPIE",

number = "8",

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TY - JOUR

T1 - Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels

AU - Pu, Yang

AU - Xue, Jianpeng

AU - Wang, Wubao

AU - Xu, Baogang

AU - Gu, Yueqing

AU - Tang, Rui

AU - Ackerstaff, Ellen

AU - Koutcher, Jason A.

AU - Achilefu, Samuel

AU - Alfano, Robert R.

N1 - Funding Information: This research is supported in part by U.S. Army Medical Research and Material Command grants numbered W81XWH-11-1-0335 and W81XWH-08-1-0717, and the National Institutes of Health (NIBIB R01 EB008111 and NCI R01 CA171651). Yang Pu thanks deeply Ms. Brenda Phelps at the administration of the Optical Radiology Lab at Washington University School of Medicine for her help and support during his working on this project at Washington University. Yang Pu also acknowledges Ms. Natalia Kruchevsky at Memorial Sloan Kettering Cancer Center (MSKCC) for her help in cell culture techniques.

PY - 2013/8

Y1 - 2013/8

N2 - The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells.

AB - The spectral changes of native fluorophores among normal fibroblasts and cancer cell lines of different metastatic ability are investigated by fluorescence spectroscopy. The normal (fibroblast), moderately metastatic (DU-145), and advanced metastatic (PC-3) cell lines were each selectively excited at 300 nm, and their fluorescence emission spectra are analyzed using principal component analysis to explore the differences of the relative contents of tryptophan and reduced nicotinamide adenine dinucleotide in these cell lines. The results show that the tryptophan emission featured predominantly in the fluorescence spectra of the advanced metastatic cancer cells in comparison with the moderately metastatic cancer and normal cells.

KW - NADH

KW - metastasisrelevant key fluorophores

KW - moderately metastatic and advanced metastatic cell lines

KW - native fluorescence spectra

KW - normal

KW - optical biopsy

KW - tryptophan

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ER -

Native fluorescence spectroscopy reveals spectral differences among prostate cancer cell lines with different risk levels

Abstract

Keywords

ASJC Scopus subject areas

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