Natural antibodies to the human T cell lymphoma virus in patients with cutaneous T cell lymphomas

L. E. Posner, M. Robert-Guroff, V. S. Kalyanaraman, B. J. Poiesz, F. W. Ruscetti, B. Fossieck, P. A. Bunn, J. D. Minna, R. C. Gallo

Research output: Contribution to journalArticle

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Abstract

Sera from patients with cutaneous T cell lymphoma and leukemia were screened for the presence of natural antibody to the human T cell lymphoma (leukemia) virus, HTLV(CR), using a solid-phase radioimmunoassay. Sera from two patients, including patient CR, from whose cultured T lymphoblastic cell line (HUT102) the retrovirus HTLV(CR) was isolated, reacted specifically with proteins of HTLV(CR). SErum from patient CR also reacted specifically with proteins of HTLV(MB), an independent but highly related retroviral isolate from a patient with Sezary T cell leukemia. The specificity for HTLV(CR) proteins was demostrated by solid-phase immunocompetition assays and competition radioimmunoprecipitation assays. Analysis of radioimmunoprecipitates indicated that the natural antibodies were directed against HTLV(CR) core proteins with molecular weights of 24,000 and 19,000 (p24 and p19). Whereas the serum reactivities for HTLV(CR) proteins were shown to be highly specific, additional reactivities seen against proteins of animal retroviruses including GaLV, SSV, FeLV, and BaEV were clearly shown not to be viral preparations or with related antigens present in fetal calf serum. These results demonstrating natural antibodies to HTLV(CR) provide the first evidence for a specific antiody response to a retrovirus in humans.

Original languageEnglish (US)
Pages (from-to)333-346
Number of pages14
JournalJournal of Experimental Medicine
Volume154
Issue number2
DOIs
StatePublished - 1981

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Cutaneous T-Cell Lymphoma
T-Cell Lymphoma
Viruses
Deltaretrovirus Antibodies
Antibodies
T-Cell Leukemia
Serum
Retroviridae
Proteins
Retroviridae Proteins
Radioimmunoprecipitation Assay
Feline Leukemia Virus
Radioimmunoassay
Molecular Weight
Antigens
Cell Line

ASJC Scopus subject areas

  • Immunology

Cite this

Posner, L. E., Robert-Guroff, M., Kalyanaraman, V. S., Poiesz, B. J., Ruscetti, F. W., Fossieck, B., ... Gallo, R. C. (1981). Natural antibodies to the human T cell lymphoma virus in patients with cutaneous T cell lymphomas. Journal of Experimental Medicine, 154(2), 333-346. https://doi.org/10.1084/jem.154.2.333

Natural antibodies to the human T cell lymphoma virus in patients with cutaneous T cell lymphomas. / Posner, L. E.; Robert-Guroff, M.; Kalyanaraman, V. S.; Poiesz, B. J.; Ruscetti, F. W.; Fossieck, B.; Bunn, P. A.; Minna, J. D.; Gallo, R. C.

In: Journal of Experimental Medicine, Vol. 154, No. 2, 1981, p. 333-346.

Research output: Contribution to journalArticle

Posner, LE, Robert-Guroff, M, Kalyanaraman, VS, Poiesz, BJ, Ruscetti, FW, Fossieck, B, Bunn, PA, Minna, JD & Gallo, RC 1981, 'Natural antibodies to the human T cell lymphoma virus in patients with cutaneous T cell lymphomas', Journal of Experimental Medicine, vol. 154, no. 2, pp. 333-346. https://doi.org/10.1084/jem.154.2.333
Posner LE, Robert-Guroff M, Kalyanaraman VS, Poiesz BJ, Ruscetti FW, Fossieck B et al. Natural antibodies to the human T cell lymphoma virus in patients with cutaneous T cell lymphomas. Journal of Experimental Medicine. 1981;154(2):333-346. https://doi.org/10.1084/jem.154.2.333
Posner, L. E. ; Robert-Guroff, M. ; Kalyanaraman, V. S. ; Poiesz, B. J. ; Ruscetti, F. W. ; Fossieck, B. ; Bunn, P. A. ; Minna, J. D. ; Gallo, R. C. / Natural antibodies to the human T cell lymphoma virus in patients with cutaneous T cell lymphomas. In: Journal of Experimental Medicine. 1981 ; Vol. 154, No. 2. pp. 333-346.
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