Natural killer T cells recognize diacylglycerol antigens from pathogenic bacteria

Yuki Kinjo; Emmanuel Tupin; Douglass Wu; Masakazu Fujio; Raquel Garcia-Navarro; Mohammed Rafii El Idrissi Benhnia; Dirk M. Zajonc; Gil Ben-Menachem; Gary D. Ainge; Gavin F. Painter; Archana Khurana; Kasper Hoebe; Samuel M. Behar; Bruce Beutler; Ian A. Wilson; Moriya Tsuji; Timothy J. Sellati; Chi Huey Wong; Mitchell Kronenberg

doi:10.1038/ni1380

Natural killer T cells recognize diacylglycerol antigens from pathogenic bacteria

Yuki Kinjo, Emmanuel Tupin, Douglass Wu, Masakazu Fujio, Raquel Garcia-Navarro, Mohammed Rafii El Idrissi Benhnia, Dirk M. Zajonc, Gil Ben-Menachem, Gary D. Ainge, Gavin F. Painter, Archana Khurana, Kasper Hoebe, Samuel M. Behar, Bruce Beutler, Ian A. Wilson, Moriya Tsuji, Timothy J. Sellati, Chi Huey Wong, Mitchell Kronenberg

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543 Scopus citations

Abstract

Natural killer T (NKT) cells recognize glycosphingolipids presented by CD1d molecules and have been linked to defense against microbial infections. Previously defined foreign glycosphingolipids recognized by NKT cells are uniquely found in nonpathogenic sphingomonas bacteria. Here we show that mouse and human NKT cells also recognized glycolipids, specifically a diacylglycerol, from Borrelia burgdorferi, which causes Lyme disease. The B. burgdorferi-derived, glycolipid-induced NKT cell proliferation and cytokine production and the antigenic potency of this glycolipid was dependent on acyl chain length and saturation. These data indicate that NKT cells recognize categories of glycolipids beyond those in sphingomonas and suggest that NKT cell responses driven by T cell receptor-mediated glycolipid recognition may provide protection against diverse pathogens.

Original language	English (US)
Pages (from-to)	978-986
Number of pages	9
Journal	Nature immunology
Volume	7
Issue number	9
DOIs	https://doi.org/10.1038/ni1380
State	Published - Sep 2006

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1038/ni1380

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Kinjo, Y., Tupin, E., Wu, D., Fujio, M., Garcia-Navarro, R., Benhnia, M. R. E. I., Zajonc, D. M., Ben-Menachem, G., Ainge, G. D., Painter, G. F., Khurana, A., Hoebe, K., Behar, S. M., Beutler, B., Wilson, I. A., Tsuji, M., Sellati, T. J., Wong, C. H., & Kronenberg, M. (2006). Natural killer T cells recognize diacylglycerol antigens from pathogenic bacteria. Nature immunology, 7(9), 978-986. https://doi.org/10.1038/ni1380

Kinjo, Y, Tupin, E, Wu, D, Fujio, M, Garcia-Navarro, R, Benhnia, MREI, Zajonc, DM, Ben-Menachem, G, Ainge, GD, Painter, GF, Khurana, A, Hoebe, K, Behar, SM, Beutler, B, Wilson, IA, Tsuji, M, Sellati, TJ, Wong, CH & Kronenberg, M 2006, 'Natural killer T cells recognize diacylglycerol antigens from pathogenic bacteria', Nature immunology, vol. 7, no. 9, pp. 978-986. https://doi.org/10.1038/ni1380

@article{6bf7f421fc7a44b68e20d38552a157bb,

title = "Natural killer T cells recognize diacylglycerol antigens from pathogenic bacteria",

abstract = "Natural killer T (NKT) cells recognize glycosphingolipids presented by CD1d molecules and have been linked to defense against microbial infections. Previously defined foreign glycosphingolipids recognized by NKT cells are uniquely found in nonpathogenic sphingomonas bacteria. Here we show that mouse and human NKT cells also recognized glycolipids, specifically a diacylglycerol, from Borrelia burgdorferi, which causes Lyme disease. The B. burgdorferi-derived, glycolipid-induced NKT cell proliferation and cytokine production and the antigenic potency of this glycolipid was dependent on acyl chain length and saturation. These data indicate that NKT cells recognize categories of glycolipids beyond those in sphingomonas and suggest that NKT cell responses driven by T cell receptor-mediated glycolipid recognition may provide protection against diverse pathogens.",

author = "Yuki Kinjo and Emmanuel Tupin and Douglass Wu and Masakazu Fujio and Raquel Garcia-Navarro and Benhnia, {Mohammed Rafii El Idrissi} and Zajonc, {Dirk M.} and Gil Ben-Menachem and Ainge, {Gary D.} and Painter, {Gavin F.} and Archana Khurana and Kasper Hoebe and Behar, {Samuel M.} and Bruce Beutler and Wilson, {Ian A.} and Moriya Tsuji and Sellati, {Timothy J.} and Wong, {Chi Huey} and Mitchell Kronenberg",

note = "Funding Information: We thank M.J. Caimano (University of Connecticut Health Science Center, Farmington, Connecticut) for B. burgdorferi–infected ticks; E. Janssen and S. McBride (La Jolla Institute for Allergy & Immunology, La Jolla, California) for TrifLps2/Lps2 mice; V. Kumar (Torrey Pines Institute for Molecular Studies, San Diego, California) for the sulfatide-reactive T cell hybridoma; N. Nagarajan, R. Severins, Y.W. Zhu (La Jolla Institute for Allergy & Immunology, La Jolla, California) and P. Rogers (Gemini Science, San Diego, California) for technical assistance; and G. Kim (La Jolla Institute for Allergy & Immunology, La Jolla, California) and M.A. Poles (New York University School of Medicine, New York, New York) for suggestions. Supported by the National Institutes of Health (AI45053 and AI71922 to M.K.; GM62116 to M.K. and I.A.W.; GM44154 to C.-H.W.; AI054546 to T.J.S.; CA58896 to I.A.W.; and AI062842 to M.T.), the Arthritis Foundation (T.J.S.) and the Cancer Research Institute (Y.K.).",

year = "2006",

month = sep,

doi = "10.1038/ni1380",

language = "English (US)",

volume = "7",

pages = "978--986",

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T1 - Natural killer T cells recognize diacylglycerol antigens from pathogenic bacteria

AU - Kinjo, Yuki

AU - Tupin, Emmanuel

AU - Wu, Douglass

AU - Fujio, Masakazu

AU - Garcia-Navarro, Raquel

AU - Benhnia, Mohammed Rafii El Idrissi

AU - Zajonc, Dirk M.

AU - Ben-Menachem, Gil

AU - Ainge, Gary D.

AU - Painter, Gavin F.

AU - Khurana, Archana

AU - Hoebe, Kasper

AU - Behar, Samuel M.

AU - Beutler, Bruce

AU - Wilson, Ian A.

AU - Tsuji, Moriya

AU - Sellati, Timothy J.

AU - Wong, Chi Huey

AU - Kronenberg, Mitchell

N1 - Funding Information: We thank M.J. Caimano (University of Connecticut Health Science Center, Farmington, Connecticut) for B. burgdorferi–infected ticks; E. Janssen and S. McBride (La Jolla Institute for Allergy & Immunology, La Jolla, California) for TrifLps2/Lps2 mice; V. Kumar (Torrey Pines Institute for Molecular Studies, San Diego, California) for the sulfatide-reactive T cell hybridoma; N. Nagarajan, R. Severins, Y.W. Zhu (La Jolla Institute for Allergy & Immunology, La Jolla, California) and P. Rogers (Gemini Science, San Diego, California) for technical assistance; and G. Kim (La Jolla Institute for Allergy & Immunology, La Jolla, California) and M.A. Poles (New York University School of Medicine, New York, New York) for suggestions. Supported by the National Institutes of Health (AI45053 and AI71922 to M.K.; GM62116 to M.K. and I.A.W.; GM44154 to C.-H.W.; AI054546 to T.J.S.; CA58896 to I.A.W.; and AI062842 to M.T.), the Arthritis Foundation (T.J.S.) and the Cancer Research Institute (Y.K.).

PY - 2006/9

Y1 - 2006/9

N2 - Natural killer T (NKT) cells recognize glycosphingolipids presented by CD1d molecules and have been linked to defense against microbial infections. Previously defined foreign glycosphingolipids recognized by NKT cells are uniquely found in nonpathogenic sphingomonas bacteria. Here we show that mouse and human NKT cells also recognized glycolipids, specifically a diacylglycerol, from Borrelia burgdorferi, which causes Lyme disease. The B. burgdorferi-derived, glycolipid-induced NKT cell proliferation and cytokine production and the antigenic potency of this glycolipid was dependent on acyl chain length and saturation. These data indicate that NKT cells recognize categories of glycolipids beyond those in sphingomonas and suggest that NKT cell responses driven by T cell receptor-mediated glycolipid recognition may provide protection against diverse pathogens.

AB - Natural killer T (NKT) cells recognize glycosphingolipids presented by CD1d molecules and have been linked to defense against microbial infections. Previously defined foreign glycosphingolipids recognized by NKT cells are uniquely found in nonpathogenic sphingomonas bacteria. Here we show that mouse and human NKT cells also recognized glycolipids, specifically a diacylglycerol, from Borrelia burgdorferi, which causes Lyme disease. The B. burgdorferi-derived, glycolipid-induced NKT cell proliferation and cytokine production and the antigenic potency of this glycolipid was dependent on acyl chain length and saturation. These data indicate that NKT cells recognize categories of glycolipids beyond those in sphingomonas and suggest that NKT cell responses driven by T cell receptor-mediated glycolipid recognition may provide protection against diverse pathogens.

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SP - 978

EP - 986

JO - Nature immunology

JF - Nature immunology

IS - 9

ER -

Natural killer T cells recognize diacylglycerol antigens from pathogenic bacteria

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