Nestin promoter/enhancer directs transgene expression to precursors of adult generated periglomerular neurons

Robert D. Beech, Muriel A. Cleary, Helen B. Treloar, Amelia J. Eisch, Alexia V. Harrist, Weimin Zhong, Charles A. Greer, Ronald S. Duman, Marina R. Picciotto

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

The subventricular zone (SVZ) is a major neurogenic region in the adult brain. Cells from the SVZ give rise to two populations of olfactory bulb interneurons: the granule cells and periglomerular (PG) cells. Currently, little is known about the signaling pathways that direct these newly generated neurons to become either granule or PG neurons. In the present study, we used the nestin promoter and enhancer to direct expression of the tetracycline transactivator (tTA). We generated two independent strains of nestin-tTA transgenic animals and crossed founder mice from both lines to mice containing a tetracycline-regulated transgene (mCREB) whose expression served as a marker for the activity of the nestin-tTA transgene. mCREB expression occurred in a subset of proliferating cells in the SVZ and rostral migratory stream in both lines. Surprisingly, in both lines of nestin-tTA mice transgene expression in the olfactory bulb was limited to PG neurons and was absent from granule cells, suggesting that this nestin promoter construct differentiates between the two interneuronal populations. Transgene expression occurred in several subtypes of PG neurons, including those expressing calretinin, calbindin, GAD67, and tyrosine hydroxylase. These results suggest that a unique subset of SVZ precursor cells gives rise to PG, and not granule cells. The ability to express different transgenes within this subpopulation of neuronal precursors provides a powerful system to define the signals regulating the differentiation and survival of adult-generated neurons in the olfactory bulb.

Original languageEnglish (US)
Pages (from-to)128-141
Number of pages14
JournalJournal of Comparative Neurology
Volume475
Issue number1
DOIs
StatePublished - Jul 12 2004

Fingerprint

Nestin
Transgenes
Tetracycline
Trans-Activators
Neurons
Lateral Ventricles
Olfactory Bulb
Calbindin 2
Calbindins
Genetically Modified Animals
Tyrosine 3-Monooxygenase
Interneurons
Population
Brain

Keywords

  • Adult neurogenesis
  • CREB
  • Inducible expression
  • MCREB
  • Olfactory bulb
  • Subventricular zone
  • Tetracycline transactivator

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Nestin promoter/enhancer directs transgene expression to precursors of adult generated periglomerular neurons. / Beech, Robert D.; Cleary, Muriel A.; Treloar, Helen B.; Eisch, Amelia J.; Harrist, Alexia V.; Zhong, Weimin; Greer, Charles A.; Duman, Ronald S.; Picciotto, Marina R.

In: Journal of Comparative Neurology, Vol. 475, No. 1, 12.07.2004, p. 128-141.

Research output: Contribution to journalArticle

Beech, RD, Cleary, MA, Treloar, HB, Eisch, AJ, Harrist, AV, Zhong, W, Greer, CA, Duman, RS & Picciotto, MR 2004, 'Nestin promoter/enhancer directs transgene expression to precursors of adult generated periglomerular neurons', Journal of Comparative Neurology, vol. 475, no. 1, pp. 128-141. https://doi.org/10.1002/cne.20179
Beech, Robert D. ; Cleary, Muriel A. ; Treloar, Helen B. ; Eisch, Amelia J. ; Harrist, Alexia V. ; Zhong, Weimin ; Greer, Charles A. ; Duman, Ronald S. ; Picciotto, Marina R. / Nestin promoter/enhancer directs transgene expression to precursors of adult generated periglomerular neurons. In: Journal of Comparative Neurology. 2004 ; Vol. 475, No. 1. pp. 128-141.
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AU - Harrist, Alexia V.

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AB - The subventricular zone (SVZ) is a major neurogenic region in the adult brain. Cells from the SVZ give rise to two populations of olfactory bulb interneurons: the granule cells and periglomerular (PG) cells. Currently, little is known about the signaling pathways that direct these newly generated neurons to become either granule or PG neurons. In the present study, we used the nestin promoter and enhancer to direct expression of the tetracycline transactivator (tTA). We generated two independent strains of nestin-tTA transgenic animals and crossed founder mice from both lines to mice containing a tetracycline-regulated transgene (mCREB) whose expression served as a marker for the activity of the nestin-tTA transgene. mCREB expression occurred in a subset of proliferating cells in the SVZ and rostral migratory stream in both lines. Surprisingly, in both lines of nestin-tTA mice transgene expression in the olfactory bulb was limited to PG neurons and was absent from granule cells, suggesting that this nestin promoter construct differentiates between the two interneuronal populations. Transgene expression occurred in several subtypes of PG neurons, including those expressing calretinin, calbindin, GAD67, and tyrosine hydroxylase. These results suggest that a unique subset of SVZ precursor cells gives rise to PG, and not granule cells. The ability to express different transgenes within this subpopulation of neuronal precursors provides a powerful system to define the signals regulating the differentiation and survival of adult-generated neurons in the olfactory bulb.

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