Neural and non-neural control of skin blood flow during isometric handgrip exercise in the heat stressed human

Manabu Shibasaki, Peter Rasmussen, Niels H. Secher, Craig G. Crandall

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

During heat stress, isometric handgrip (IHG) exercise causes cutaneous vasoconstriction, but it remains controversial whether neural mechanisms are responsible for this observation. The objective of this study was to test the hypothesis that cutaneous vasoconstriction during IHG exercise in heat stressed individuals occurs via a neural mechanism. An axillary nerve blockade was performed to block efferent nerve traffic to the left forearm in seven healthy subjects. Two intradermal microdialysis probes were placed within forearm skin of the blocked area. Forearm skin blood flow was measured by laser-Doppler flowmetry over the microdialysis probes as well as from skin of the contralateral (unblocked) forearm. Cutaneous vascular conductance (CVC) was calculated from the ratio of skin blood flow to mean arterial pressure. Effectiveness of nerve blockade was verified by the absence of tactile sensation, as well as an absence of sweating and cutaneous vasodilatation during a whole-body heat stress. Upon this confirmation, adenosine was perfused through one of the microdialysis probes to increase skin blood flow similar to that of the unblocked site. After internal temperature increased ∼0.7°C, subjects performed 2 min of IHG exercise at 35% of maximal voluntary contraction using the non-blocked arm. IHG exercise significantly decreased CVC at the unblocked site (82.3 ± 5.7 to 70.9 ± 5.4%max, P = 0.005, means ± s.e.m.) and the adenosine treated site of the blocked arm (75.2 ± 7.2 to 68.3 ± 6.6%max, P = 0.005), whereas CVC was unchanged at the blocked site that did not receive adenosine (15.7 ± 2.8 to 13.7 ± 2.0%max, P = 0.10). Importantly, the reduction in CVC was greater at the unblocked site than at the adenosine treated site (11.4 ± 2.6 vs. 6.9 ± 1.6% max, respectively, P = 0.01). These findings suggest that neural and non-neural mechanisms contribute to the reduction in forearm CVC during IHG exercise in heat stressed humans.

Original languageEnglish (US)
Pages (from-to)2101-2107
Number of pages7
JournalJournal of Physiology
Volume587
Issue number9
DOIs
StatePublished - 2009

Fingerprint

Hot Temperature
Exercise
Skin
Forearm
Blood Vessels
Adenosine
Nerve Block
Microdialysis
Vasoconstriction
Arm
Laser-Doppler Flowmetry
Sweating
Touch
Vasodilation
Healthy Volunteers
Arterial Pressure
Temperature

ASJC Scopus subject areas

  • Physiology

Cite this

Neural and non-neural control of skin blood flow during isometric handgrip exercise in the heat stressed human. / Shibasaki, Manabu; Rasmussen, Peter; Secher, Niels H.; Crandall, Craig G.

In: Journal of Physiology, Vol. 587, No. 9, 2009, p. 2101-2107.

Research output: Contribution to journalArticle

Shibasaki, Manabu ; Rasmussen, Peter ; Secher, Niels H. ; Crandall, Craig G. / Neural and non-neural control of skin blood flow during isometric handgrip exercise in the heat stressed human. In: Journal of Physiology. 2009 ; Vol. 587, No. 9. pp. 2101-2107.
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abstract = "During heat stress, isometric handgrip (IHG) exercise causes cutaneous vasoconstriction, but it remains controversial whether neural mechanisms are responsible for this observation. The objective of this study was to test the hypothesis that cutaneous vasoconstriction during IHG exercise in heat stressed individuals occurs via a neural mechanism. An axillary nerve blockade was performed to block efferent nerve traffic to the left forearm in seven healthy subjects. Two intradermal microdialysis probes were placed within forearm skin of the blocked area. Forearm skin blood flow was measured by laser-Doppler flowmetry over the microdialysis probes as well as from skin of the contralateral (unblocked) forearm. Cutaneous vascular conductance (CVC) was calculated from the ratio of skin blood flow to mean arterial pressure. Effectiveness of nerve blockade was verified by the absence of tactile sensation, as well as an absence of sweating and cutaneous vasodilatation during a whole-body heat stress. Upon this confirmation, adenosine was perfused through one of the microdialysis probes to increase skin blood flow similar to that of the unblocked site. After internal temperature increased ∼0.7°C, subjects performed 2 min of IHG exercise at 35{\%} of maximal voluntary contraction using the non-blocked arm. IHG exercise significantly decreased CVC at the unblocked site (82.3 ± 5.7 to 70.9 ± 5.4{\%}max, P = 0.005, means ± s.e.m.) and the adenosine treated site of the blocked arm (75.2 ± 7.2 to 68.3 ± 6.6{\%}max, P = 0.005), whereas CVC was unchanged at the blocked site that did not receive adenosine (15.7 ± 2.8 to 13.7 ± 2.0{\%}max, P = 0.10). Importantly, the reduction in CVC was greater at the unblocked site than at the adenosine treated site (11.4 ± 2.6 vs. 6.9 ± 1.6{\%} max, respectively, P = 0.01). These findings suggest that neural and non-neural mechanisms contribute to the reduction in forearm CVC during IHG exercise in heat stressed humans.",
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