Neural crest stem cells persist in the adult gut but undergo changes in self-renewal, neuronal subtype potential, and factor responsiveness

Genevieve M. Kruger, Jack T. Mosher, Suzanne Bixby, Nancy Joseph, Toshihide Iwashita, Sean J. Morrison

Research output: Contribution to journalArticle

411 Citations (Scopus)

Abstract

We found neural crest stem cells (NCSCs) in the adult gut. Postnatal gut NCSCs were isolated by flow-cytometry and compared to fetal gut NCSCs. They self-renewed extensively in culture but less than fetal gut NCSCs. Postnatal gut NCSCs made neurons that expressed a variety of neurotransmitters but lost the ability to make certain subtypes of neurons that are generated during fetal development. Postnatal gut NCSCs also differed in their responsiveness to lineage determination factors, affecting cell fate determination in vivo and possibly explaining their reduced neuronal subtype potential. These perinatal changes in gut NCSCs parallel perinatal changes in hematopoietic stem cells, suggesting that stem cells in different tissues undergo similar developmental transitions. The persistence of NCSCs in the adult PNS opens up new possibilities for regeneration after injury or disease.

Original languageEnglish (US)
Pages (from-to)657-669
Number of pages13
JournalNeuron
Volume35
Issue number4
DOIs
StatePublished - Aug 15 2002

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Neural Stem Cells
Neural Crest
Neurons
Fetal Development
Hematopoietic Stem Cells
Neurotransmitter Agents
Regeneration
Flow Cytometry
Stem Cells
Wounds and Injuries

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neural crest stem cells persist in the adult gut but undergo changes in self-renewal, neuronal subtype potential, and factor responsiveness. / Kruger, Genevieve M.; Mosher, Jack T.; Bixby, Suzanne; Joseph, Nancy; Iwashita, Toshihide; Morrison, Sean J.

In: Neuron, Vol. 35, No. 4, 15.08.2002, p. 657-669.

Research output: Contribution to journalArticle

Kruger, Genevieve M. ; Mosher, Jack T. ; Bixby, Suzanne ; Joseph, Nancy ; Iwashita, Toshihide ; Morrison, Sean J. / Neural crest stem cells persist in the adult gut but undergo changes in self-renewal, neuronal subtype potential, and factor responsiveness. In: Neuron. 2002 ; Vol. 35, No. 4. pp. 657-669.
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