Neural crest stem cells undergo multilineage differentiation in developing peripheral nerves to generate endoneurial fibroblasts in addition to Schwann cells

Nancy M. Joseph, Yoh Suke Mukouyama, Jack T. Mosher, Martine Jaegle, Steven A. Crone, Emma Louise Dormand, Kuo Fen Lee, Dies Meijer, David J. Anderson, Sean J. Morrison

Research output: Contribution to journalArticle

210 Citations (Scopus)

Abstract

Neural crest stem cells (NCSCs) persist in peripheral nerves throughout late gestation but their function is unknown. Current models of nerve development only consider the generation of Schwann cells from neural crest, but the presence of NCSCs raises the possibility of multilineage differentiation. We performed Cre-recombinase fate mapping to determine which nerve cells are neural crest derived. Endoneurial fibroblasts, in addition to myelinating and non-myelinating Schwann cells, were neural crest derived, whereas perineurial cells, pericytes and endothelial cells were not. This identified endoneurial fibroblasts as a novel neural crest derivative, and demonstrated that trunk neural crest does give rise to fibroblasts in vivo, consistent with previous studies of trunk NCSCs in culture. The multilineage differentiation of NCSCs into glial and non-glial derivatives in the developing nerve appears to be regulated by neuregulin, notch ligands, and bone morphogenic proteins, as these factors are expressed in the developing nerve, and cause nerve NCSCs to generate Schwann cells and fibroblasts, but not neurons, in culture. Nerve development is thus more complex than was previously thought, involving NCSC self-renewal, lineage commitment and multilineage differentiation.

Original languageEnglish (US)
Pages (from-to)5599-5612
Number of pages14
JournalDevelopment
Volume131
Issue number22
DOIs
StatePublished - Nov 2004

Fingerprint

Neural Stem Cells
Neural Crest
Schwann Cells
Peripheral Nerves
Fibroblasts
Neuregulins
Neurons
Pericytes
Neuroglia
Endothelial Cells
Cell Culture Techniques
Ligands
Bone and Bones

Keywords

  • Fate-mapping
  • Neural crest stem cell
  • Peripheral nerve development

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

Neural crest stem cells undergo multilineage differentiation in developing peripheral nerves to generate endoneurial fibroblasts in addition to Schwann cells. / Joseph, Nancy M.; Mukouyama, Yoh Suke; Mosher, Jack T.; Jaegle, Martine; Crone, Steven A.; Dormand, Emma Louise; Lee, Kuo Fen; Meijer, Dies; Anderson, David J.; Morrison, Sean J.

In: Development, Vol. 131, No. 22, 11.2004, p. 5599-5612.

Research output: Contribution to journalArticle

Joseph, NM, Mukouyama, YS, Mosher, JT, Jaegle, M, Crone, SA, Dormand, EL, Lee, KF, Meijer, D, Anderson, DJ & Morrison, SJ 2004, 'Neural crest stem cells undergo multilineage differentiation in developing peripheral nerves to generate endoneurial fibroblasts in addition to Schwann cells', Development, vol. 131, no. 22, pp. 5599-5612. https://doi.org/10.1242/dev.01429
Joseph, Nancy M. ; Mukouyama, Yoh Suke ; Mosher, Jack T. ; Jaegle, Martine ; Crone, Steven A. ; Dormand, Emma Louise ; Lee, Kuo Fen ; Meijer, Dies ; Anderson, David J. ; Morrison, Sean J. / Neural crest stem cells undergo multilineage differentiation in developing peripheral nerves to generate endoneurial fibroblasts in addition to Schwann cells. In: Development. 2004 ; Vol. 131, No. 22. pp. 5599-5612.
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