Neurofibromatosis Type 2 (NF2) Gene Is Somatically Mutated in Mesothelioma but not in Lung Cancer

Y. Sekido, H. I. Pass, S. Bader, D. J Y Mew, M. F. Christman, A. F. Gazdar, J. D. Minna

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239 Scopus citations

Abstract

We have found 16 of 28 small cell lung cancers, 17 of 31 non-small cell lung cancers, 2 of 3 carcinoids, and 12 of 14 mesotheliomas that had chromosome 22 cytogenetic abnormalities. To determine whether the neurofibromatosis type 2 (NF2) gene located on chromosome 22 participates in the oncogenesis of these malignancies, we studied DNAs from lung cancer cell lines and mesotheliomas using Southern blot analysis and the single-strand conformation polymorphism (SSCP) technique for mutations covering 8 of the 16 known NF2 exons. We detected 7 mutations in 17 mesotheliomas (41%) within the coding region of NF2 but none in 75 lung cancer cell lines (38 small cell lung cancers, 34 non-small cell lung cancers, and 3 carcinoids). These mutations were found to be somatic when normal tissue was available for testing. Four mesothelioma cell lines had relatively large deletions (~10–50 kilobases) in the NF2 gene detectable by Southern blot analysis. Two mesothelioma cell lines had nonsense mutations at codons 57 and 341, respectively. Another mesothelioma obtained as a specimen directly from a patient, had a 10-base pair microdeletion from nucleotide 1004 to nucleotide 1013 causing a frame-shift mutation. These results suggest that the NF2 gene participates in the oncogenesis in a subset of mesotheliomas but not in lung cancers.

Original languageEnglish (US)
Pages (from-to)1227-1231
Number of pages5
JournalCancer research
Volume55
Issue number6
StatePublished - Mar 15 1995

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Sekido, Y., Pass, H. I., Bader, S., Mew, D. J. Y., Christman, M. F., Gazdar, A. F., & Minna, J. D. (1995). Neurofibromatosis Type 2 (NF2) Gene Is Somatically Mutated in Mesothelioma but not in Lung Cancer. Cancer research, 55(6), 1227-1231.