Neuromedin B is present in lung cancer cell lines

G. Giaccone, J. Battey, A. F. Gazdar, H. Oie, M. Draoui, T. W. Moody

Research output: Contribution to journalArticle

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Abstract

Previously, high levels of gastrin-releasing peptide and its mRNA were detected in classic small cell lung cancer cell lines. Here the ability of lung cancer cell lines to synthesize neuromedin B (NMB), a structurally similar mammalian bombesin-like peptide, was investigated. By radioimmunoassay, NMB (0.1-0.7 pmol/mg of protein) was detected in 23 of 33 lung cancer cell lines. In contrast, gastrin-releasing peptide (0.1-12.9 pmol/mg of protein) was detected in 16 of 32 cell lines. Using gel filtration and high pressure liquid chromatography techniques, the main peak of immunoreactive NMB coeluted with synthetic NMB. By Northern analysis, a 0.8-kilobase mRNA species was present, using poly(A) mRNA derived from two of three lung cancer cell lines. Using a more sensitive S1 nuclease protection assay, NMB mRNA was present in most of the 15 lung cancer cell lines examined. These data suggest that NMB may be a regulatory peptide in lung cancer.

Original languageEnglish (US)
JournalCancer Research
Volume52
Issue number9 SUPPL.
StatePublished - May 1 1992

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Lung Neoplasms
Cell Line
Gastrin-Releasing Peptide
Messenger RNA
Nuclease Protection Assays
Bombesin
Peptides
Small Cell Lung Carcinoma
Radioimmunoassay
Gel Chromatography
neuromedin B
Proteins
High Pressure Liquid Chromatography

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Giaccone, G., Battey, J., Gazdar, A. F., Oie, H., Draoui, M., & Moody, T. W. (1992). Neuromedin B is present in lung cancer cell lines. Cancer Research, 52(9 SUPPL.).

Neuromedin B is present in lung cancer cell lines. / Giaccone, G.; Battey, J.; Gazdar, A. F.; Oie, H.; Draoui, M.; Moody, T. W.

In: Cancer Research, Vol. 52, No. 9 SUPPL., 01.05.1992.

Research output: Contribution to journalArticle

Giaccone, G, Battey, J, Gazdar, AF, Oie, H, Draoui, M & Moody, TW 1992, 'Neuromedin B is present in lung cancer cell lines', Cancer Research, vol. 52, no. 9 SUPPL..
Giaccone G, Battey J, Gazdar AF, Oie H, Draoui M, Moody TW. Neuromedin B is present in lung cancer cell lines. Cancer Research. 1992 May 1;52(9 SUPPL.).
Giaccone, G. ; Battey, J. ; Gazdar, A. F. ; Oie, H. ; Draoui, M. ; Moody, T. W. / Neuromedin B is present in lung cancer cell lines. In: Cancer Research. 1992 ; Vol. 52, No. 9 SUPPL.
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