Neuromedin B is present in lung cancer cell lines

G. Giaccone, J. Battey, A. F. Gazdar, H. Oie, M. Draoui, T. W. Moody

Research output: Contribution to journalArticle

69 Scopus citations

Abstract

Previously, high levels of gastrin-releasing peptide and its mRNA were detected in classic small cell lung cancer cell lines. Here the ability of lung cancer cell lines to synthesize neuromedin B (NMB), a structurally similar mammalian bombesin-like peptide, was investigated. By radioimmunoassay, NMB (0.1-0.7 pmol/mg of protein) was detected in 23 of 33 lung cancer cell lines. In contrast, gastrin-releasing peptide (0.1-12.9 pmol/mg of protein) was detected in 16 of 32 cell lines. Using gel filtration and high pressure liquid chromatography techniques, the main peak of immunoreactive NMB coeluted with synthetic NMB. By Northern analysis, a 0.8- kilobase mRNA species was present, using poly(A) mRNA derived from two of three lung cancer cell lines. Using a more sensitive S1 nuclease protection assay, NMB mRNA was present in most of the 15 lung cancer cell lines examined. These data suggest that NMB may be a regulatory peptide in lung cancer.

Original languageEnglish (US)
Pages (from-to)2732s-2736s
JournalCancer research
Volume52
Issue number9 SUPPL.
StatePublished - Jan 1 1992

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Neuromedin B is present in lung cancer cell lines'. Together they form a unique fingerprint.

  • Cite this

    Giaccone, G., Battey, J., Gazdar, A. F., Oie, H., Draoui, M., & Moody, T. W. (1992). Neuromedin B is present in lung cancer cell lines. Cancer research, 52(9 SUPPL.), 2732s-2736s.