Neuromuscular electrical stimulation promotes development in mice of mature human muscle from immortalized human myoblasts

Paraskevi Sakellariou, Andrea O'Neill, Amber L. Mueller, Guido Stadler, Woodring E. Wright, Joseph A. Roche, Robert J. Bloch

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Studies of the pathogenic mechanisms underlying human myopathies and muscular dystrophies often require animal models, but models of some human diseases are not yet available. Methods to promote the engraftment and development of myogenic cells from individuals with such diseases in mice would accelerate such studies and also provide a useful tool for testing therapeutics. Here, we investigate the ability of immortalized human myogenic precursor cells (hMPCs) to form mature human myofibers following implantation into the hindlimbs of non-obese diabetic-Rag1 null IL2rγ null (NOD-Rag)-immunodeficient mice. Results: We report that hindlimbs of NOD-Rag mice that are X-irradiated, treated with cardiotoxin, and then injected with immortalized control hMPCs or hMPCs from an individual with facioscapulohumeral muscular dystrophy (FSHD) develop mature human myofibers. Furthermore, intermittent neuromuscular electrical stimulation (iNMES) of the peroneal nerve of the engrafted limb enhances the development of mature fibers in the grafts formed by both immortal cell lines. With control cells, iNMES increases the number and size of the human myofibers that form and promotes closer fiber-to-fiber packing. The human myofibers in the graft are innervated, fully differentiated, and minimally contaminated with murine myonuclei. Conclusions: Our results indicate that control and FSHD human myofibers can form in mice engrafted with hMPCs and that iNMES enhances engraftment and subsequent development of mature human muscle.

Original languageEnglish (US)
Article number4
JournalSkeletal Muscle
Volume6
Issue number1
DOIs
StatePublished - Feb 27 2016

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Myoblasts
Electric Stimulation
Muscles
Facioscapulohumeral Muscular Dystrophy
Hindlimb
Cardiotoxins
Transplants
Peroneal Nerve
Aptitude
Muscular Dystrophies
Human Development
Muscular Diseases
Extremities
Animal Models
Cell Line

Keywords

  • Desmin
  • Differentiation
  • FSHD
  • Lamin
  • Muscular dystrophy
  • Neuromuscular junction
  • Spectrin
  • Stem cell
  • Xenograft

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Orthopedics and Sports Medicine

Cite this

Neuromuscular electrical stimulation promotes development in mice of mature human muscle from immortalized human myoblasts. / Sakellariou, Paraskevi; O'Neill, Andrea; Mueller, Amber L.; Stadler, Guido; Wright, Woodring E.; Roche, Joseph A.; Bloch, Robert J.

In: Skeletal Muscle, Vol. 6, No. 1, 4, 27.02.2016.

Research output: Contribution to journalArticle

Sakellariou, Paraskevi ; O'Neill, Andrea ; Mueller, Amber L. ; Stadler, Guido ; Wright, Woodring E. ; Roche, Joseph A. ; Bloch, Robert J. / Neuromuscular electrical stimulation promotes development in mice of mature human muscle from immortalized human myoblasts. In: Skeletal Muscle. 2016 ; Vol. 6, No. 1.
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