Neuronal properties of hybrid neuroblastoma x sympathetic ganglion cells

L. A. Greene, W. Shain, A. Chalazonitis, X. Breakfield, J. Minna, H. G. Coon, M. Nirenberg

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

Clonal mouse neuroblastoma cells without tyrosine 3 monoxygenase [EC 1.14.16.2; tyrosine hydroxylase; L tyrosine, tetrahydropteridine:oxygen oxidoreductase (3 hydroxylating)] activity were fused with normal cells from embryonic mouse sympathetic ganglia. One of the 37 hybrid cell lines obtained possesses high tyrosine 3 monooxygenase activity and synthesizes dopamine. These cells also have excitable membranes and generate action potentials in response to electrical stimuli. Thus hybrid cells, generated by fusion of neuroblastoma cells with normal cells from the nervous system, can acquire neural properties not found with the parental neuroblastoma cells.

Original languageEnglish (US)
Pages (from-to)4923-4927
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume72
Issue number12
DOIs
StatePublished - Dec 1 1975

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