Neuropeptide Y deficiency attenuates responses to fasting and high-fat diet in obesity-prone mice

Hiralben R. Patel, Yong Qi, Evan J. Hawkins, Stanley M. Hileman, Joel K. Elmquist, Yumi Imai, Rexford S. Ahima

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Neuropeptide Y (NPY) stimulates feeding and weight gain, but deletion of the NPY gene does not affect food intake and body weight in mice bred on a mixed genetic background. We reasoned that the orexigenic action of NPY would be evident in C57Bl/6J mice susceptible to obesity. NPY deficiency has no significant effect in mice fed a normal rodent diet. However, energy expenditure is elevated during fasting, and hyperphagia and weight gain are blunted during refeeding. Expression of agouti-related peptide (AGRP) in the hypothalamus is increased in NPY knockout (NPYko) than wild-type mice, but unlike wild type there is no further increase in AGRP when NPYko mice are fasted. Moreover, NPYko mice have higher oxygen consumption and uncoupling protein-1 expression in brown adipose tissue during fasting. The failure of an increase in orexigenic peptides and higher thermogenesis may contribute to attenuation of weight gain when NPYko mice are refed. C57Bl/6J mice lacking NPY are also less susceptible to diet-induced obesity (DIO) as a result of reduced feeding and increased energy expenditure. The resistance to DIO in NPYko mice is associated with a reduction in nocturnal feeding and increased expression of anorexigenic hypothalamic peptides. Insulin, leptin, and triglyceride levels increase with adiposity in both wild-type and NPYko mice.

Original languageEnglish (US)
Pages (from-to)3091-3098
Number of pages8
JournalDiabetes
Volume55
Issue number11
DOIs
StatePublished - Nov 2006

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Neuropeptide Y
High Fat Diet
Knockout Mice
Fasting
Obesity
Weight Gain
Diet
Energy Metabolism
Peptides
Hyperphagia
Brown Adipose Tissue
Thermogenesis
Adiposity
Leptin
Oxygen Consumption
Hypothalamus
Rodentia
Triglycerides
Eating
Body Weight

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Patel, H. R., Qi, Y., Hawkins, E. J., Hileman, S. M., Elmquist, J. K., Imai, Y., & Ahima, R. S. (2006). Neuropeptide Y deficiency attenuates responses to fasting and high-fat diet in obesity-prone mice. Diabetes, 55(11), 3091-3098. https://doi.org/10.2337/db05-0624

Neuropeptide Y deficiency attenuates responses to fasting and high-fat diet in obesity-prone mice. / Patel, Hiralben R.; Qi, Yong; Hawkins, Evan J.; Hileman, Stanley M.; Elmquist, Joel K.; Imai, Yumi; Ahima, Rexford S.

In: Diabetes, Vol. 55, No. 11, 11.2006, p. 3091-3098.

Research output: Contribution to journalArticle

Patel, HR, Qi, Y, Hawkins, EJ, Hileman, SM, Elmquist, JK, Imai, Y & Ahima, RS 2006, 'Neuropeptide Y deficiency attenuates responses to fasting and high-fat diet in obesity-prone mice', Diabetes, vol. 55, no. 11, pp. 3091-3098. https://doi.org/10.2337/db05-0624
Patel, Hiralben R. ; Qi, Yong ; Hawkins, Evan J. ; Hileman, Stanley M. ; Elmquist, Joel K. ; Imai, Yumi ; Ahima, Rexford S. / Neuropeptide Y deficiency attenuates responses to fasting and high-fat diet in obesity-prone mice. In: Diabetes. 2006 ; Vol. 55, No. 11. pp. 3091-3098.
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