Neuropil pattern formation and regulation of cell adhesion molecules in Drosophila optic lobe development depend on synaptobrevin

Peter Robin Hiesinger, Christian Reiter, Harald Schau, Karl Friedrich Fischbach

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

To investigate a possible involvement of synaptic machinery in Drosophila visual system development, we studied the effects of a loss of function of neuronal synaptobrevin, a protein required for synaptic vesicle release. Expression of tetanus toxin light chain (which cleaves neuronal synaptobrevin) and genetic mosaics were used to analyze neuropil pattern formation and levels of selected neural adhesion molecules in the optic lobe. We show that targeted toxin expression in the developing optic lobe results in disturbances of the columnar organization of visual neuropils and of photoreceptor terminal morphology. IrreC-rst immunoreactivity in neuropils is increased after widespread expression of toxin. In photoreceptors, targeted toxin expression results in increased Fasciclin II and chaoptin but not IrreC-rst immunoreactivity. Axonal pathfinding and programmed cell death are not affected. In genetic mosaics, patches of photoreceptors that lack neuronal synaptobrevin exhibit the same phenotypes observed after photoreceptor-specific toxin expression. Our results demonstrate the requirement of neuronal synaptobrevin for regulation of cell adhesion molecules and development of the fine structure of the optic lobe. A possible causal link to fine-tuning processes that may include synaptic plasticity in the development of the Drosophila CNS is discussed.

Original languageEnglish (US)
Pages (from-to)7548-7556
Number of pages9
JournalJournal of Neuroscience
Volume19
Issue number17
StatePublished - Sep 1 1999

Fingerprint

R-SNARE Proteins
Neuropil
Cell Adhesion Molecules
Drosophila
Immunotoxins
Tetanus Toxin
Neuronal Plasticity
Synaptic Vesicles
Cell Death
Phenotype
Light
Proteins

Keywords

  • Cell adhesion molecules
  • Chaoptin
  • Drosophila
  • Fasciclin II
  • IrreC-rst
  • Optic lobe development
  • Synaptic plasticity
  • Synaptobrevin
  • Tetanus toxin

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neuropil pattern formation and regulation of cell adhesion molecules in Drosophila optic lobe development depend on synaptobrevin. / Hiesinger, Peter Robin; Reiter, Christian; Schau, Harald; Fischbach, Karl Friedrich.

In: Journal of Neuroscience, Vol. 19, No. 17, 01.09.1999, p. 7548-7556.

Research output: Contribution to journalArticle

Hiesinger, Peter Robin ; Reiter, Christian ; Schau, Harald ; Fischbach, Karl Friedrich. / Neuropil pattern formation and regulation of cell adhesion molecules in Drosophila optic lobe development depend on synaptobrevin. In: Journal of Neuroscience. 1999 ; Vol. 19, No. 17. pp. 7548-7556.
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