Neuroprotective effect of prenyloxycoumarins from edible vegetables

Francesco Epifano, Gemma Molinaro, Salvatore Genovese, Richard Teke Ngomba, Ferdinando Nicoletti, Massimo Curini

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

The present study is designed to investigate the effect of some natural prenyloxyphenylpropanoids as neuroprotective agents against NMDA-induced toxicity in mixed cortical cell cultures containing both neurons and astrocytes. Excitotoxicity was induced by exposure of cultures to NMDA (100 μM) at room temperature in a HEPES-buffered salt solution followed by incubation at 37 °C for the following 24 h in MEM-Eagle's supplemented with 15.8 mM NaHCO3 and 25 mM glucose. Tested compounds were mixed with NMDA. Neuronal injury was measured in all experiments by examination of cultures with phase-contrast microscopy at 20×, 18-20 h after the insult while neuronal damage was quantitatively assessed by counting dead neurons stained with trypan blue and by measuring lactate dehydrogenase (LDH) released in the medium. Results showed that only natural prenyloxyphenylpropanoids containing a coumarin nucleus, namely 7-isopentenyloxycoumarin and auraptene, both found in nature from plants belonging to the genus Citrus and other of the family of Rutaceae, including edible ones, exerted a good dose-dependent manner protective effect against NMDA-induced neurotoxicity in particular at concentrations ranging from 1 to 10 μM.

Original languageEnglish (US)
Pages (from-to)57-60
Number of pages4
JournalNeuroscience Letters
Volume443
Issue number2
DOIs
StatePublished - Oct 3 2008

Fingerprint

Neuroprotective Agents
N-Methylaspartate
Vegetables
Rutaceae
HEPES
Neurons
Phase-Contrast Microscopy
Eagles
Trypan Blue
Citrus
L-Lactate Dehydrogenase
Astrocytes
Cell Culture Techniques
Salts
Glucose
Temperature
5-hydroxy-6-methoxy-7-(3-methyl-but-2-enyloxy)-2H-1-benzopyran-2-one
Wounds and Injuries

Keywords

  • Citrus spp.
  • Mixed cortical cultures
  • Neuroprotective activity
  • Prenyloxycoumarins

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Epifano, F., Molinaro, G., Genovese, S., Ngomba, R. T., Nicoletti, F., & Curini, M. (2008). Neuroprotective effect of prenyloxycoumarins from edible vegetables. Neuroscience Letters, 443(2), 57-60. https://doi.org/10.1016/j.neulet.2008.07.062

Neuroprotective effect of prenyloxycoumarins from edible vegetables. / Epifano, Francesco; Molinaro, Gemma; Genovese, Salvatore; Ngomba, Richard Teke; Nicoletti, Ferdinando; Curini, Massimo.

In: Neuroscience Letters, Vol. 443, No. 2, 03.10.2008, p. 57-60.

Research output: Contribution to journalArticle

Epifano, F, Molinaro, G, Genovese, S, Ngomba, RT, Nicoletti, F & Curini, M 2008, 'Neuroprotective effect of prenyloxycoumarins from edible vegetables', Neuroscience Letters, vol. 443, no. 2, pp. 57-60. https://doi.org/10.1016/j.neulet.2008.07.062
Epifano, Francesco ; Molinaro, Gemma ; Genovese, Salvatore ; Ngomba, Richard Teke ; Nicoletti, Ferdinando ; Curini, Massimo. / Neuroprotective effect of prenyloxycoumarins from edible vegetables. In: Neuroscience Letters. 2008 ; Vol. 443, No. 2. pp. 57-60.
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