Neurotrophin 3 stimulates the differentiation of motoneurons from avian neural tube progenitor cells

Lea Averbuch-Heller, Merav Pruginin, Nitza Kahane, Pantelis Tsoulfas, Luis Parada, Arnon Rosenthal, Chaya Kalcheim

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Neurotrophin 3 (NT-3) promotes differentiation of neural tube progenitors into motoneurons expressing the BEN/SC1 and islet-1 epitopes. A 1.75- to 6.7- fold increase in BEN-positive motoneurons was obtained when quail neural tube cells were cultured with NT-3 at 0.1-10 ng/ml, respectively. In contrast, the overall number of cells, as well as the proportion of motoneurons that developed from cycling precursors, did not change. Addition of NT-3 at 1 ng/ml to cells obtained from ventral half-neural tubes promoted a 2.5-fold stimulation in motoneuron number, confirming the specificity of the effect. Moreover, NT-3 had no significant effect on survival of differentiated avian motoneurons. The distribution of trkC mRNA, which encodes the high-affinity receptor for NT-3, is consistent with these findings. trkC expression is homogeneous in the embryonic day 2 (E2) neural tube, becomes restricted to the mantle layer on E3, where differentiation occurs, and disappears from the ventral third of the E4-E5 spinal cord right before the onset of normal motoneuron death. These results suggest that NT-3 and trkC regulate early neurogenesis in the avian central nervous system.

Original languageEnglish (US)
Pages (from-to)3247-3251
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number8
StatePublished - Apr 12 1994

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Neurotrophin 3
Neural Tube
Motor Neurons
Stem Cells
trkC Receptor
Quail
Neurogenesis
Epitopes
Cultured Cells
Spinal Cord
Central Nervous System
Cell Count
Messenger RNA

Keywords

  • avian embryo
  • survival
  • trophic factors
  • tyrosine kinase receptor

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Averbuch-Heller, L., Pruginin, M., Kahane, N., Tsoulfas, P., Parada, L., Rosenthal, A., & Kalcheim, C. (1994). Neurotrophin 3 stimulates the differentiation of motoneurons from avian neural tube progenitor cells. Proceedings of the National Academy of Sciences of the United States of America, 91(8), 3247-3251.

Neurotrophin 3 stimulates the differentiation of motoneurons from avian neural tube progenitor cells. / Averbuch-Heller, Lea; Pruginin, Merav; Kahane, Nitza; Tsoulfas, Pantelis; Parada, Luis; Rosenthal, Arnon; Kalcheim, Chaya.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, No. 8, 12.04.1994, p. 3247-3251.

Research output: Contribution to journalArticle

Averbuch-Heller, L, Pruginin, M, Kahane, N, Tsoulfas, P, Parada, L, Rosenthal, A & Kalcheim, C 1994, 'Neurotrophin 3 stimulates the differentiation of motoneurons from avian neural tube progenitor cells', Proceedings of the National Academy of Sciences of the United States of America, vol. 91, no. 8, pp. 3247-3251.
Averbuch-Heller, Lea ; Pruginin, Merav ; Kahane, Nitza ; Tsoulfas, Pantelis ; Parada, Luis ; Rosenthal, Arnon ; Kalcheim, Chaya. / Neurotrophin 3 stimulates the differentiation of motoneurons from avian neural tube progenitor cells. In: Proceedings of the National Academy of Sciences of the United States of America. 1994 ; Vol. 91, No. 8. pp. 3247-3251.
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