New class of polyomavirus mutant that can persist as free copies in F9 embryonal carcinoma cells.

K. Ariizumi, H. Ariga

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A small circular DNA was found extrachromosomally in a clone of F9 embryonal carcinoma (EC) cells at high copy numbers per cell. The DNA was cloned in plasmid pUC19. Restriction endonuclease analyses of the DNA indicated that the DNA (fPyF9) was a mutant of polyomavirus (Py) DNA and had a mutation in a noncoding regulatory region. There have been many reports on the isolation of Py mutants capable of replication in undifferentiated cells. However, fPyF9 was different from other Py mutants in the following aspects: it was harbored stably as a free copy at 1 X 10(4) to 5 X 10(4) copies per cell in EC cells; it replicated in undifferentiated cells better than in differentiated cells; it was extremely rearranged in the sequences of the enhancer B domain; and it carried in the enhancer B domain three copies of an exogenous sequence which does not exist in Py strain A2. From these observations, we propose a new class of Py EC mutant which has an autonomous state similar to that of plasmid and small circular DNA in host cells.

Original languageEnglish (US)
Pages (from-to)3920-3927
Number of pages8
JournalMolecular and cellular biology
Volume6
Issue number11
DOIs
StatePublished - Nov 1986

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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